Ultrastructural changes and immunohistochemical localization of nitric oxide synthase, advanced glycation end products and NF-kappa B in aorta of streptozotocin treated Mongolian gerbils.

R Nishigaki, F Guo, M Onda, N Yamada, M Yokoyama, Z Naito, G Asano, M ShimizuSuganuma, K Shichinohe, T Aramaki
{"title":"Ultrastructural changes and immunohistochemical localization of nitric oxide synthase, advanced glycation end products and NF-kappa B in aorta of streptozotocin treated Mongolian gerbils.","authors":"R Nishigaki,&nbsp;F Guo,&nbsp;M Onda,&nbsp;N Yamada,&nbsp;M Yokoyama,&nbsp;Z Naito,&nbsp;G Asano,&nbsp;M ShimizuSuganuma,&nbsp;K Shichinohe,&nbsp;T Aramaki","doi":"10.1272/jnms.66.166","DOIUrl":null,"url":null,"abstract":"<p><p>To evaluate the relationship among the induction of nitric oxide synthase (NOS), advanced glycation end products (AGEs) and NF-kappa B for vascular damage in hyperglycemia, we injected Mongolian gerbils intravenously with 150 mg/kg streptozotocin (STZ) and observed over the next one year the resulting aortic changes by immunohistochemical and electron microscopical techniques. After STZ treatment, hyperglycemia was confirmed and body weight transiently decreased. Morphological observation revealed no remarkable changs in vascular endothelial cells or vascular smooth muscle cells in the aorta at one week after STZ administration. After 4 weeks increased collagen fibrils were observed in the pericellular spaces of media. At one year after STZ administration, increased collagen fibrils and thickened elastic fibers were found around the vascular smooth muscle cells with vacuolization and increased cytoplasmic organellae compared with non-treated animals of the same age. Immunohistochemically endothelial constitutive NOS (ecNOS) was localized in the endothelium of the aorta of Mongolian gerbils. At one year after STZ administration, the reaction products of iNOS, AGEs and NF-kappa B in vascular endothelial cells and smooth muscle cells were much more greatly increased than at one week and 4 weeks. After STZ administration, the localization of NOS, AGEs and NF-kappa B was observed in the aorta, which suggests these factors play important roles in the pathogenesis of vasculopathy in diabetes mellitus.</p>","PeriodicalId":19192,"journal":{"name":"Nihon Ika Daigaku zasshi","volume":"66 3","pages":"166-75"},"PeriodicalIF":0.0000,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"18","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nihon Ika Daigaku zasshi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1272/jnms.66.166","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 18

Abstract

To evaluate the relationship among the induction of nitric oxide synthase (NOS), advanced glycation end products (AGEs) and NF-kappa B for vascular damage in hyperglycemia, we injected Mongolian gerbils intravenously with 150 mg/kg streptozotocin (STZ) and observed over the next one year the resulting aortic changes by immunohistochemical and electron microscopical techniques. After STZ treatment, hyperglycemia was confirmed and body weight transiently decreased. Morphological observation revealed no remarkable changs in vascular endothelial cells or vascular smooth muscle cells in the aorta at one week after STZ administration. After 4 weeks increased collagen fibrils were observed in the pericellular spaces of media. At one year after STZ administration, increased collagen fibrils and thickened elastic fibers were found around the vascular smooth muscle cells with vacuolization and increased cytoplasmic organellae compared with non-treated animals of the same age. Immunohistochemically endothelial constitutive NOS (ecNOS) was localized in the endothelium of the aorta of Mongolian gerbils. At one year after STZ administration, the reaction products of iNOS, AGEs and NF-kappa B in vascular endothelial cells and smooth muscle cells were much more greatly increased than at one week and 4 weeks. After STZ administration, the localization of NOS, AGEs and NF-kappa B was observed in the aorta, which suggests these factors play important roles in the pathogenesis of vasculopathy in diabetes mellitus.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
链脲佐菌素对蒙古沙鼠主动脉一氧化氮合酶、晚期糖基化终产物和nf - κ B的超微结构变化和免疫组化定位。
为了探讨一氧化氮合酶(NOS)、晚期糖基化终产物(AGEs)和NF-kappa B在高血糖血管损伤中的作用,我们给蒙古沙鼠静脉注射了150 mg/kg链脲佐菌素(STZ),并通过免疫组化和电镜技术观察了随后一年的主动脉变化。经STZ治疗后,血糖升高,体重短暂下降。STZ给药1周后,形态学观察显示主动脉血管内皮细胞和血管平滑肌细胞无明显变化。4周后,中膜细胞周间隙胶原原纤维增多。STZ给药1年后,血管平滑肌细胞周围胶原原纤维增多,弹性纤维增厚,空泡化,细胞器增多。免疫组化法检测到蒙古沙鼠主动脉内皮内皮构成性NOS (ecNOS)。STZ给药1年后,血管内皮细胞和平滑肌细胞中iNOS、AGEs和nf - κ B的反应产物明显高于给药1周和4周时。STZ给药后主动脉内NOS、AGEs和nf - κ B的定位,提示这些因子在糖尿病血管病变的发病机制中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
[Magnetic resonance imaging]. Risk factors and pathogenesis of atherosclerotic lesion. The short-term effects of tamsulosin in Japanese men with benign prostatic hyperplasia. [QT dispersion in premature beats produced by extrastimuli from the right atrium and right ventricle]. [Cardiac catheterization by a transradial approach].
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1