Development of reversed passive latex agglutination for detection of Thai cobra (Naja kaouthia) venom.

Journal of natural toxins Pub Date : 1999-06-01
O Khow, N Wongtongkam, N Pakmanee, T Omori-Satoh, V Sitprija
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引用次数: 0

Abstract

A simple, rapid, and sensitive diagnostic kit for detecting Thai cobra (Naja kaouthia) venom was developed using latex particles sensitized with venom specific immunoglobulin. The kit is capable of detecting 25-50 ng/ml of Thai cobra venom. The capability was not affected by human plasma. Specificity of the kit was proven using snake venoms from Vipera russelli, Calloselasma rhodostoma, Trimeresurus albolabris, Naja siamensis, Ophiophagus hannah, and Bungarus fasciatus. The diagnostic kit does not lose its capability under refrigeration for two months and by lyophilization.

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反向被动乳胶凝集检测泰国眼镜蛇毒液的研究进展。
研制了一种简单、快速、灵敏的泰国眼镜蛇(Naja kaouthia)毒液检测试剂盒,该试剂盒采用毒液特异性免疫球蛋白致敏乳胶颗粒。该试剂盒能够检测25-50 ng/ml的泰国眼镜蛇毒液。这种能力不受人体血浆的影响。该试剂盒的特异性被证明是由蛇的毒液,从蝰蛇russelli, Calloselasma rhodostoma, Trimeresurus albolabris, Naja siamensis, Ophiophagus hannah和Bungarus fasciatus。该诊断试剂盒在冷冻两个月和冻干后不会失去其功能。
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