Heat stable lipase activity of thermotolerant bacteria from hot springs at Orissa, India.

Cytobios Pub Date : 1999-01-01
C C Rath
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Abstract

Thermotolerant bacteria (35 in toto) isolated from three hot springs (Atri, Taptapani and Deuljhari, Orissa), were screened for lipase activities. Of these, nine strains of Bacillus spp. and three strains of Pseudomonas spp. showed heat stable lipase activity at 60 degrees C. The hydrolytic activity of these bacteria was tested using Tween-20 and Tween-80 as substrates at different temperatures using plate assay and titration techniques. The hydrolytic activity at different pH values and salt concentrations was investigated.

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印度奥里萨邦温泉中耐热细菌的热稳定脂肪酶活性。
从3个温泉(Atri, Taptapani和Deuljhari, Orissa)中分离到35株耐热菌,对其脂肪酶活性进行了筛选。其中,9株芽孢杆菌和3株假单胞菌在60℃时表现出热稳定脂肪酶活性。以Tween-20和Tween-80为底物,采用平板法和滴定法测定了这些细菌在不同温度下的水解活性。考察了不同pH值和盐浓度下的水解活性。
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