Comparison of three typing methods for Pseudomonas aeruginosa.

Abstract

Fifty-seven independent isolates of Pseudomonas aeruginosa from blood specimens were typed with 3 different methods: ribotyping, random amplified polymorphic DNA (RAPD) typing, and pyocin typing. Ribotyping was performed by probing the rRNA genes of genomic DNA that was digested separately with 4 different restriction enzymes. Digestion of DNA from 57 P. aeruginosa isolates with BamHI, ClaI, EcoRI, and PstI produced 4, 4, 6, and 7 patterns, respectively. As a result, ribotyping classified the 57 isolates into 22 types. Six new ribotypes that had not been described previously were found. One BamHI, 1 ClaI, 2 EcoRI, and 2 PstI patterns were novel. RAPD typing was performed with two different polymerase chain reaction (PCR) primers (RAPD1 and RAPD2). Both primers classified the 57 isolates into 15 RAPD types and produced identical patterns. The pyocin typing method classified the 57 isolates into 10 types. According to the results obtained in this study, the ribotyping has a discriminatory index of 0.865, RAPD, 0.785, and pyocin typing, 0.676, respectively. The ribotyping method was the most effective among the 3 methods compared for typing P. aeruginosa isolates.