Effects of MK-447 on platelet shape change, aggregation, and ATP release by collagen, ADP, and stable analogue of thromboxane A2 in rabbit platelets.

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-07-01

B Y Li, H Zhou, G F Qiao, L Wang, W H Li

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Abstract

Aim: To investigate the effects of MK-447 on platelet shape change, aggregation, and ATP release by collagen (Col), ADP, and stable analogue of thromboxane A2 (STA2) in rabbits.

Methods: Platelet shape change and aggregation were quantified in light transmission by turbidimetric method and release reaction was assessed by the amount of ATP in platelet-rich plasma (PRP).

Results: (1) MK-447 100-700 mumol.L-1 caused only the shape change, which was not inhibited by indometacin 3 mumol.L-1. Platelet shape changes by Col, ADP, and STA2 were reduced (P < 0.01) after the addition of MK-447. The lag phase was prolonged (P < 0.01) in Col and shortened (P < 0.01) in ADP. (2) MK-447 reduced the aggregation by Col 5 mg.L-1 (P < 0.01), and enhanced that by ADP 0.3-10 mumol.L-1 and STA2 0.1-3 mumol.L-1 (P < 0.01). (3) The release reaction by STA2 1-3 mumol.L-1 was also increased (P < 0.01). The effects of MK-447 on STA2 were not inhibited by S-145.

Conclusion: MK-447 induced the platelet shape change, and showed the dual effects, inhibition or enhancement, on the actions by different aggregating agents.

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MK-447对兔血小板中胶原、ADP和稳定类似物血栓素A2的血小板形状改变、聚集和ATP释放的影响

目的:探讨MK-447对兔血小板形状改变、聚集及胶原蛋白(Col)、ADP和稳定类似物血栓素A2 (STA2)释放ATP的影响。方法:采用透光比浊法测定血小板形状变化和聚集，富血小板血浆(PRP)中ATP含量测定血小板释放反应。结果:(1)MK-447 100-700;L-1仅引起形状变化，吲哚美辛3mol - L-1对其无抑制作用。加入MK-447后，Col、ADP和STA2对血小板形状的影响均降低(P < 0.01)。Col组滞后期延长(P < 0.01)， ADP组滞后期缩短(P < 0.01)。(2) MK-447降低了col5 mg的聚集。L-1 (P < 0.01)，且ADP 0.3 ~ 10 μ mol使其增强。L-1和STA2 0.1-3 μ mol。L-1 (p < 0.01)。(3) STA2 1-3 mumol的释放反应。L-1也显著升高(P < 0.01)。MK-447对STA2的影响不受S-145的抑制。结论:MK-447可诱导血小板形态改变，并对不同聚集剂的作用表现出抑制或增强的双重作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zhongguo yao li xue bao = Acta pharmacologica Sinica

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