{"title":"Presence of SSAO in human and bovine meninges and microvessels.","authors":"V Castillo, J M Lizcano, M Unzeta","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>In spite that SSAO enzyme is widely distributed in almost all tissues, specially in vascularized ones, its presence in brain microvessels is still controversy. Our results resolve this question showing that both human and bovine cerebrovascular tissues do contain the SSAO enzyme. This was achieved biochemically, using benzylamine and methylamine as substrates, and by immunoblot analysis, using polyclonal antibodies anti-SSAO that recognized a 100 kDa single band in tissue homogenates.</p>","PeriodicalId":79356,"journal":{"name":"Neurobiology (Budapest, Hungary)","volume":"7 3","pages":"263-72"},"PeriodicalIF":0.0000,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurobiology (Budapest, Hungary)","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
In spite that SSAO enzyme is widely distributed in almost all tissues, specially in vascularized ones, its presence in brain microvessels is still controversy. Our results resolve this question showing that both human and bovine cerebrovascular tissues do contain the SSAO enzyme. This was achieved biochemically, using benzylamine and methylamine as substrates, and by immunoblot analysis, using polyclonal antibodies anti-SSAO that recognized a 100 kDa single band in tissue homogenates.
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