Cellulase hyperproducers constructed from polyploids of Lentinus edodes.

Microbios Pub Date : 2000-01-01
H Toyama, N Toyama
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Abstract

A mycelial mat of Lentinus edodes was treated with 0.01% (w/v) colchicine solution for 240 h at 26 degrees C and autopolyploidization occurred. The mycelia were treated subsequently with the haploidizing reagent, benomyl, and fanshaped sectors were produced from colonies. Among such sectors, cellulase hyperproducers could be selected. The cellulase productivity of the hyperproducer, L1, did not decrease through five generations.

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利用香菇多倍体构建纤维素酶高产体。
用0.01% (w/v)秋水仙碱溶液在26℃下处理香菇菌丝垫240 h,可发生自多倍体化。随后用单倍体化试剂处理菌丝,得到苯甲酰,菌落产生扇形扇形部门。在这些部门中,可以选择纤维素酶超级生产商。高产菌株L1的纤维素酶产率在五代中没有下降。
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