Sister chromatid exchange in chromosomes of sheep (Ovis aries).

Cytobios Pub Date : 2000-01-01
G P Di Meo, A Perucatti, D Fornataro, D Incarnato, L Ferrara, D Matassino, L Iannuzzi
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Abstract

Blood lymphocyte cultures from 32 Comisana and Laticauda sheep breeds (15 males and 17 females) raised in Southern Italy were studied using sister chromatid exchange (SCE) test. Of the 932 cells studied, the SCE-mean value was 7.20 +/- 2.5 per cell for both breeds. Indeed, the SCE mean values were 7.12 +/- 2.45 and 7.28 +/- 2.55 in Comisana and Laticauda breeds, respectively, and the differences were not significant. No statistical differences were noticed between male and female cells (7.25 +/- 2.39 and 7.16 +/- 2.60, respectively). The SCE frequency distribution did not follow a Poisson distribution. The number of SCE were significantly higher than expected in chromosomes 1, 2 and 3 (p < 0.001) and significantly lower than expected in the X and remaining chromosomes (p < 0.001) on the basis of relative chromosome lengths.

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绵羊染色体中的姐妹染色单体交换。
采用姐妹染色单体交换法(SCE)对意大利南部饲养的32个科米萨纳羊和拉蒂库达羊品种(15个公羊和17个母羊)的血淋巴细胞培养进行了研究。在所研究的932个细胞中,两个品种的sce平均值为每细胞7.20 +/- 2.5。Comisana和Laticauda品种的SCE平均值分别为7.12 +/- 2.45和7.28 +/- 2.55,差异不显著。雌雄细胞间无统计学差异(分别为7.25 +/- 2.39和7.16 +/- 2.60)。SCE频率分布不服从泊松分布。根据相对染色体长度,1、2、3号染色体的SCE数量显著高于预期(p < 0.001),而X和其余染色体的SCE数量显著低于预期(p < 0.001)。
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