Disruption of peripheral target contact influences the development of identified central dendritic branches in a leech motor neuron in vivo.

Journal of neurobiology Pub Date : 2000-06-15
L A Johnson, W B Kristan, J Jellies, K A French
{"title":"Disruption of peripheral target contact influences the development of identified central dendritic branches in a leech motor neuron in vivo.","authors":"L A Johnson,&nbsp;W B Kristan,&nbsp;J Jellies,&nbsp;K A French","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Retrograde signaling from target tissues has been shown to influence many aspects of neuronal development in a number of developmental systems. In these experiments using embryonic leeches (Hirudo medicinalis), we examined how depriving a neuron of contact with its peripheral target affects the development of the cell's central arborization. We focused our attention on the motor neuron cell 3, which normally stimulates dorsal longitudinal muscle fibers to contract. At different locations in the periphery and in embryos of several different stages, we cut the nerve containing the growing axon of cell 3. This surgery led to dramatic overgrowth of cell 3's central dendritic branches, which normally accept synaptic contacts from other neurons, including the inhibitory motor neuron cell 1. When cell 3's peripheral axon was cut relatively early in development, its overgrown central branches eventually retracted. However, cells that were disrupted later in development retained their overextended branches into adulthood. In addition, if the axon was cut close to the ganglion early in development, depriving the cell of contact with any dorsal tissues, the central branches failed to retract and were instead retained into adulthood. Unlike cell 3, the central branches of cell 1, which has the same peripheral target muscles as cell 3, remained unchanged following all axotomy protocols. These results suggest that in at least some neurons contact with peripheral targets can influence development of the central processes that normally mediate synaptic contacts.</p>","PeriodicalId":16540,"journal":{"name":"Journal of neurobiology","volume":"43 4","pages":"365-78"},"PeriodicalIF":0.0000,"publicationDate":"2000-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of neurobiology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Retrograde signaling from target tissues has been shown to influence many aspects of neuronal development in a number of developmental systems. In these experiments using embryonic leeches (Hirudo medicinalis), we examined how depriving a neuron of contact with its peripheral target affects the development of the cell's central arborization. We focused our attention on the motor neuron cell 3, which normally stimulates dorsal longitudinal muscle fibers to contract. At different locations in the periphery and in embryos of several different stages, we cut the nerve containing the growing axon of cell 3. This surgery led to dramatic overgrowth of cell 3's central dendritic branches, which normally accept synaptic contacts from other neurons, including the inhibitory motor neuron cell 1. When cell 3's peripheral axon was cut relatively early in development, its overgrown central branches eventually retracted. However, cells that were disrupted later in development retained their overextended branches into adulthood. In addition, if the axon was cut close to the ganglion early in development, depriving the cell of contact with any dorsal tissues, the central branches failed to retract and were instead retained into adulthood. Unlike cell 3, the central branches of cell 1, which has the same peripheral target muscles as cell 3, remained unchanged following all axotomy protocols. These results suggest that in at least some neurons contact with peripheral targets can influence development of the central processes that normally mediate synaptic contacts.

分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
外周目标接触的中断影响水蛭运动神经元中央树突状分支的发育。
来自目标组织的逆行信号已被证明影响许多发育系统中神经元发育的许多方面。在这些使用胚胎水蛭(Hirudo medicinalis)的实验中,我们研究了剥夺神经元与其周围靶点的接触如何影响细胞中央树突的发育。我们把注意力集中在运动神经元细胞3上,它通常刺激背纵肌纤维收缩。在不同的外周位置和不同阶段的胚胎中,我们切断了含有3号细胞生长轴突的神经。手术导致细胞3的中央树突状分支急剧过度生长,该分支通常接受来自其他神经元的突触接触,包括抑制性运动神经元细胞1。当细胞3的外周轴突在发育中相对较早被切断时,其过度生长的中央分支最终缩回。然而,在发育后期被破坏的细胞在成年期保留了它们过度伸展的分支。此外,如果轴突在发育早期被切断到神经节附近,使细胞失去与任何背侧组织的接触,那么中央分支就不能缩回,而是保留到成年期。与细胞3不同的是,细胞1的中心分支与细胞3具有相同的外周靶肌,在所有的肛切开术中保持不变。这些结果表明,至少在一些神经元与外周目标的接触可以影响通常介导突触接触的中枢过程的发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
V Fenstermaker, Y Chen, A Ghosh, R Yuste. Regulation of dendritic length and branching by Semaphorin 3A, Journal of Neurobiology (2004) 58(3) 403–412 Synaptic inputs onto spiking local interneurons in crayfish are depressed by nitric oxide. Remodeling of an identified motoneuron during metamorphosis: central and peripheral actions of ecdysteroids during regression of dendrites and motor terminals. Role of Nova-1 in regulating alpha2N, a novel glycine receptor splice variant, in developing spinal cord neurons. Cloning of the cDNA and mRNA expression of CLRP, a complex leucine repeat protein of the Golgi apparatus expressed by specific neurons of the rat brain.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1