M.Teresa Miras-Portugal, Miguel Dı́az-Hernández, Rosa Gomez-Villafuerte, Javier Gualix, Enrique Castro, Jesús Pintor
{"title":"Presynaptic signalling mediated by mono- and dinucleotides in the central nervous system","authors":"M.Teresa Miras-Portugal, Miguel Dı́az-Hernández, Rosa Gomez-Villafuerte, Javier Gualix, Enrique Castro, Jesús Pintor","doi":"10.1016/S0165-1838(00)00138-7","DOIUrl":null,"url":null,"abstract":"<div><p>Synaptosomal preparations from rat midbrain exhibit specific responses to both ATP and Ap<sub>5</sub>A, which elicit a Ca<sup>2+</sup> entrance to the presynaptic terminals. Studies of isolated single terminals showed that not all the terminals contain ionotropic receptors for nucleotides, in fact only 46% of them do. Of these, 12% responded only to the dinucleotide Ap<sub>5</sub>A, and 20% to the mononucleotide ATP. At the presynaptic level, diinosine pentaphosphate, Ip<sub>5</sub>I, is a good tool to specifically block dinucleotide responses, which are inhibited at low nM concentration, versus the high μM concentrations required to block ATP responses. There is evidence for a presynaptic control of mononucleotide and dinucleotide responses, mediated by metabotropic and ionotropic receptors. Stimulation of adenosine A1 receptors increases the affinity of dinucleotide receptors by five orders of magnitude, from 30 μM to 680 pM for control and in the presence of A1 agonist, respectively.</p></div>","PeriodicalId":17228,"journal":{"name":"Journal of the autonomic nervous system","volume":"81 1","pages":"Pages 195-199"},"PeriodicalIF":0.0000,"publicationDate":"2000-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1838(00)00138-7","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the autonomic nervous system","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165183800001387","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10
Abstract
Synaptosomal preparations from rat midbrain exhibit specific responses to both ATP and Ap5A, which elicit a Ca2+ entrance to the presynaptic terminals. Studies of isolated single terminals showed that not all the terminals contain ionotropic receptors for nucleotides, in fact only 46% of them do. Of these, 12% responded only to the dinucleotide Ap5A, and 20% to the mononucleotide ATP. At the presynaptic level, diinosine pentaphosphate, Ip5I, is a good tool to specifically block dinucleotide responses, which are inhibited at low nM concentration, versus the high μM concentrations required to block ATP responses. There is evidence for a presynaptic control of mononucleotide and dinucleotide responses, mediated by metabotropic and ionotropic receptors. Stimulation of adenosine A1 receptors increases the affinity of dinucleotide receptors by five orders of magnitude, from 30 μM to 680 pM for control and in the presence of A1 agonist, respectively.