Synthesis and functional integration of a neurotransmitter receptor in isolated invertebrate axons.

G E Spencer, N I Syed, E van Kesteren, K Lukowiak, W P Geraerts, J van Minnen
{"title":"Synthesis and functional integration of a neurotransmitter receptor in isolated invertebrate axons.","authors":"G E Spencer,&nbsp;N I Syed,&nbsp;E van Kesteren,&nbsp;K Lukowiak,&nbsp;W P Geraerts,&nbsp;J van Minnen","doi":"10.1002/1097-4695(200007)44:1<72::aid-neu7>3.0.co;2-#","DOIUrl":null,"url":null,"abstract":"<p><p>Neurotransmitter receptors are considered an important class of membrane proteins that are involved in plasticity-induced changes underlying learning and memory. Recent studies, which demonstrated that the mRNAs encoding for various receptor proteins are localized to specific dendritic domains, allude toward the possibility that these membrane bound molecules may be synthesized locally. However, direct evidence for the local axonal or dendritic synthesis and functional integration of receptor proteins in either vertebrates or invertebrates is still lacking. In this study, using an invertebrate model system we provide the first direct evidence that isolated axons (in the absence of the soma) can intrinsically synthesize and functionally integrate a membrane-bound receptor protein from an axonally injected mRNA. Surgically isolated axons from identified neurons were injected with mRNA encoding a G-protein-coupled conopressin receptor. Immunocytochemical and electrophysiological techniques were used to demonstrate functional integration of the receptor protein into the membrane of the isolated axon. Ultrastructural analysis of axonal compartments revealed polyribosomes, suggesting that some components of the protein synthesizing machinery are indeed present in these extrasomal compartments. Such axonal propensity to locally synthesize and functionally insert transmitter receptors may be instrumental in plasticity induced changes, for instance those that underlie learning and memory.</p>","PeriodicalId":16540,"journal":{"name":"Journal of neurobiology","volume":"44 1","pages":"72-81"},"PeriodicalIF":0.0000,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1097-4695(200007)44:1<72::aid-neu7>3.0.co;2-#","citationCount":"86","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of neurobiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/1097-4695(200007)44:1<72::aid-neu7>3.0.co;2-#","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 86

Abstract

Neurotransmitter receptors are considered an important class of membrane proteins that are involved in plasticity-induced changes underlying learning and memory. Recent studies, which demonstrated that the mRNAs encoding for various receptor proteins are localized to specific dendritic domains, allude toward the possibility that these membrane bound molecules may be synthesized locally. However, direct evidence for the local axonal or dendritic synthesis and functional integration of receptor proteins in either vertebrates or invertebrates is still lacking. In this study, using an invertebrate model system we provide the first direct evidence that isolated axons (in the absence of the soma) can intrinsically synthesize and functionally integrate a membrane-bound receptor protein from an axonally injected mRNA. Surgically isolated axons from identified neurons were injected with mRNA encoding a G-protein-coupled conopressin receptor. Immunocytochemical and electrophysiological techniques were used to demonstrate functional integration of the receptor protein into the membrane of the isolated axon. Ultrastructural analysis of axonal compartments revealed polyribosomes, suggesting that some components of the protein synthesizing machinery are indeed present in these extrasomal compartments. Such axonal propensity to locally synthesize and functionally insert transmitter receptors may be instrumental in plasticity induced changes, for instance those that underlie learning and memory.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
孤立无脊椎动物轴突中神经递质受体的合成和功能整合。
神经递质受体被认为是一类重要的膜蛋白,参与可塑性诱导的学习和记忆变化。最近的研究表明,编码各种受体蛋白的mrna定位于特定的树突结构域,暗示这些膜结合分子可能在局部合成。然而,在脊椎动物或无脊椎动物中,局部轴突或树突合成和受体蛋白功能整合的直接证据仍然缺乏。在这项研究中,利用无脊椎动物模型系统,我们提供了第一个直接证据,证明分离的轴突(在没有体细胞的情况下)可以从轴突注射的mRNA中内在地合成和功能整合膜结合受体蛋白。从鉴定的神经元中手术分离的轴突注射编码g蛋白偶联抗压素受体的mRNA。利用免疫细胞化学和电生理技术证明受体蛋白与分离轴突膜的功能整合。轴突腔室的超微结构分析显示存在多核糖体,这表明在这些核外腔室中确实存在蛋白质合成机制的某些成分。这种轴突局部合成和功能插入传递受体的倾向可能有助于可塑性诱导的变化,例如那些作为学习和记忆基础的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
V Fenstermaker, Y Chen, A Ghosh, R Yuste. Regulation of dendritic length and branching by Semaphorin 3A, Journal of Neurobiology (2004) 58(3) 403–412 Synaptic inputs onto spiking local interneurons in crayfish are depressed by nitric oxide. Remodeling of an identified motoneuron during metamorphosis: central and peripheral actions of ecdysteroids during regression of dendrites and motor terminals. Role of Nova-1 in regulating alpha2N, a novel glycine receptor splice variant, in developing spinal cord neurons. Cloning of the cDNA and mRNA expression of CLRP, a complex leucine repeat protein of the Golgi apparatus expressed by specific neurons of the rat brain.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1