Effects of geometry of hydroxyapatite as a cell substratum in BMP-induced ectopic bone formation.

Q M Jin, H Takita, T Kohgo, K Atsumi, H Itoh, Y Kuboki
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Abstract

Three different types of porous hydroxyapatite with pore sizes of 100-200 micrometer in diameter-porous particles of hydroxyapatite (PPHAP), porous blocks of hydroxyapatite (PBHAP), and honeycomb-shaped hydroxyapatite (HCHAP)-were compared in terms of their abilities to induce osteogenesis when implanted subcutaneously with recombinant human BMP-2 into rats and extracted at 1, 2, 3, and 4 weeks. Histologically, direct bone formation occurred in PPHAP and PBHAP while only endochondral ossification took place in HCHAP. Interestingly, cartilage in the central zones and bone in the orifice zones of the tunnels of the HCHAP were observed at 2 weeks. After 3 weeks, the cartilage disappeared and bone formation occurred throughout the inner surface of the tunnels of the HCHAP, always leaving space for capillaries within the tunnels. Alkaline phosphatase activity and osteocalcin content were the highest in HCHAP among the three hydroxyapatite implants. These results clearly indicate that BMP-induced bone formation is highly dependent on the geometry of the carrier, which provides feasible structural factors for vascularization.

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羟基磷灰石作为细胞基质在bmp诱导异位骨形成中的作用。
我们比较了孔径为100-200微米的三种不同类型的多孔羟基磷灰石——多孔羟基磷灰石颗粒(PPHAP)、多孔羟基磷灰石块(phap)和蜂窝状羟基磷灰石(HCHAP)——在重组人BMP-2皮下植入大鼠并于1、2、3和4周提取时诱导成骨的能力。组织学上,PPHAP和PBHAP发生直接成骨,而HCHAP仅发生软骨内成骨。有趣的是,在2周时观察到HCHAP隧道中心区域的软骨和孔口区域的骨。3周后,软骨消失,整个HCHAP隧道内表面出现骨形成,隧道内毛细血管始终留有空间。三种羟基磷灰石种植体HCHAP的碱性磷酸酶活性和骨钙素含量最高。这些结果清楚地表明bmp诱导的骨形成高度依赖于载体的几何形状,这为血管化提供了可行的结构因素。
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