{"title":"IgM alloantibody detection by M-MPHA, a new solid-phase assay system","authors":"Tamai Toyohiro , Ishii Yasuyuki , Mazda Toshio","doi":"10.1016/S0955-3886(00)00047-3","DOIUrl":null,"url":null,"abstract":"<div><p>New IgM alloantibody detection system that uses anti-human IgM-antibody-sensitized magnetic particles mixed passive hemagglutination (IgM-M-MPHA) has been developed. IgM antibodies are well detected by direct agglutination using the saline tube test. Anti-IgM using IgM antibody detection is not used routinely. In our evaluation, IgM-M-MPHA showed good detection of IgM alloantibodies. When IgM-M-MPHA is used together with IgG-M-MPHA (anti-human IgG-antibody-sensitized magnetic particles mixed passive hemagglutination), alloantibodies can be distinguished easily whether it is IgG, IgM or both.</p></div>","PeriodicalId":80242,"journal":{"name":"Transfusion science","volume":"23 1","pages":"Pages 47-54"},"PeriodicalIF":0.0000,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0955-3886(00)00047-3","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transfusion science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0955388600000473","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8
Abstract
New IgM alloantibody detection system that uses anti-human IgM-antibody-sensitized magnetic particles mixed passive hemagglutination (IgM-M-MPHA) has been developed. IgM antibodies are well detected by direct agglutination using the saline tube test. Anti-IgM using IgM antibody detection is not used routinely. In our evaluation, IgM-M-MPHA showed good detection of IgM alloantibodies. When IgM-M-MPHA is used together with IgG-M-MPHA (anti-human IgG-antibody-sensitized magnetic particles mixed passive hemagglutination), alloantibodies can be distinguished easily whether it is IgG, IgM or both.
采用抗人IgM抗体敏化磁颗粒混合被动血凝(IgM- m - mpha)技术，研制了新型IgM同种抗体检测系统。IgM抗体可通过生理盐水试管直接凝集检测。使用IgM抗体检测抗IgM不常规使用。在我们的评估中，IgM- m - mpha显示出良好的IgM同种异体抗体检测。当IgM- m - mpha与IgG- m - mpha(抗人IgG抗体敏化磁颗粒混合被动血凝)联合使用时，无论是IgG、IgM还是两者都可以很容易地区分同种异体抗体。
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