Ionic currents of Drosophila embryonic neurons derived from selectively cultured CNS midline precursors.

Journal of neurobiology Pub Date : 2000-09-15
H Schmidt, K Lüer, W Hevers, G M Technau
{"title":"Ionic currents of Drosophila embryonic neurons derived from selectively cultured CNS midline precursors.","authors":"H Schmidt,&nbsp;K Lüer,&nbsp;W Hevers,&nbsp;G M Technau","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>In order to investigate the electrogenesis of defined cell populations, we applied an in vitro system that allows the selective culturing of individual Drosophila CNS precursors under different conditions. CNS midline (ML) precursors prepared from gastrula stage embryos gave rise to progeny cells with neuronal and glial morphology that expressed specific markers. Using whole-cell patch-clamp recordings, a detailed description of ionic currents present in this defined cell population is provided. Most ionic currents of cultured ML neurons were similar to other cultured Drosophila neurons, even though their embryonic origin is different. They displayed at least two voltage-gated potassium currents, a voltage-gated sodium, two voltage-gated calcium currents, and responded to the neurotransmitters ACh and GABA. They showed homogeneity in action potential firing properties, generating only a single spike even upon sustained depolarization. Interestingly, although the expression of the voltage-gated potassium currents appeared to be highly cell autonomous, for all other currents significant changes were observed in the presence of fiber contacts.</p>","PeriodicalId":16540,"journal":{"name":"Journal of neurobiology","volume":"44 4","pages":"392-413"},"PeriodicalIF":0.0000,"publicationDate":"2000-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of neurobiology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

In order to investigate the electrogenesis of defined cell populations, we applied an in vitro system that allows the selective culturing of individual Drosophila CNS precursors under different conditions. CNS midline (ML) precursors prepared from gastrula stage embryos gave rise to progeny cells with neuronal and glial morphology that expressed specific markers. Using whole-cell patch-clamp recordings, a detailed description of ionic currents present in this defined cell population is provided. Most ionic currents of cultured ML neurons were similar to other cultured Drosophila neurons, even though their embryonic origin is different. They displayed at least two voltage-gated potassium currents, a voltage-gated sodium, two voltage-gated calcium currents, and responded to the neurotransmitters ACh and GABA. They showed homogeneity in action potential firing properties, generating only a single spike even upon sustained depolarization. Interestingly, although the expression of the voltage-gated potassium currents appeared to be highly cell autonomous, for all other currents significant changes were observed in the presence of fiber contacts.

分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
选择性培养中枢神经系统中线前体的果蝇胚胎神经元的离子电流。
为了研究特定细胞群的电生,我们应用了一个体外系统,允许在不同条件下选择性培养单个果蝇中枢神经系统前体。从原胚期胚胎制备的中枢神经系统中线(ML)前体产生具有表达特定标记物的神经元和胶质形态的后代细胞。使用全细胞膜片钳记录,离子电流的详细描述存在于这个定义的细胞群提供。培养的ML神经元的大多数离子电流与其他培养的果蝇神经元相似,尽管它们的胚胎来源不同。它们表现出至少两个电压门控钾电流,一个电压门控钠电流,两个电压门控钙电流,并对神经递质乙酰胆碱和氨基丁酸有反应。它们在动作电位放电特性上表现出同质性,即使持续去极化也只产生单个尖峰。有趣的是,尽管电压门控钾电流的表达似乎是高度细胞自主的,但在纤维接触存在的情况下,所有其他电流的表达都发生了显著变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
V Fenstermaker, Y Chen, A Ghosh, R Yuste. Regulation of dendritic length and branching by Semaphorin 3A, Journal of Neurobiology (2004) 58(3) 403–412 Synaptic inputs onto spiking local interneurons in crayfish are depressed by nitric oxide. Remodeling of an identified motoneuron during metamorphosis: central and peripheral actions of ecdysteroids during regression of dendrites and motor terminals. Role of Nova-1 in regulating alpha2N, a novel glycine receptor splice variant, in developing spinal cord neurons. Cloning of the cDNA and mRNA expression of CLRP, a complex leucine repeat protein of the Golgi apparatus expressed by specific neurons of the rat brain.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1