{"title":"Production of Aspergillus niger catalase under various stress conditions.","authors":"J Fiedurek","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The conidia of Aspergillus niger were cultured under various stress conditions. The highest yield of catalase activity was obtained after addition 1.2 M NaCl to 6-h-old culture, which improved the activity of the enzyme by about 2.8-fold in comparison with the control medium without this depressor. A significantly lower increase (1.3- to 1.7-fold) in catalase activity was reached when spores were incubated under oxidative stress (100-200 mM H2O2) or at low pH (2 and 3). Respiratory activities in A. niger culture show important changes with the osmotic stress increase.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"49 1","pages":"43-9"},"PeriodicalIF":0.0000,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta microbiologica Polonica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The conidia of Aspergillus niger were cultured under various stress conditions. The highest yield of catalase activity was obtained after addition 1.2 M NaCl to 6-h-old culture, which improved the activity of the enzyme by about 2.8-fold in comparison with the control medium without this depressor. A significantly lower increase (1.3- to 1.7-fold) in catalase activity was reached when spores were incubated under oxidative stress (100-200 mM H2O2) or at low pH (2 and 3). Respiratory activities in A. niger culture show important changes with the osmotic stress increase.
在不同的胁迫条件下培养黑曲霉的分生孢子。在6 h的培养基中添加1.2 M NaCl后,过氧化氢酶活性最高,比不添加该抑制剂的对照培养基提高了约2.8倍。在氧化胁迫(100-200 mM H2O2)或低pH(2和3)条件下培养孢子时,过氧化氢酶活性的增加幅度较低(1.3- 1.7倍)。黑曲霉培养的呼吸活性随着渗透胁迫的增加而发生重要变化。