Production of Aspergillus niger catalase under various stress conditions.

Acta microbiologica Polonica Pub Date : 2000-01-01
J Fiedurek
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Abstract

The conidia of Aspergillus niger were cultured under various stress conditions. The highest yield of catalase activity was obtained after addition 1.2 M NaCl to 6-h-old culture, which improved the activity of the enzyme by about 2.8-fold in comparison with the control medium without this depressor. A significantly lower increase (1.3- to 1.7-fold) in catalase activity was reached when spores were incubated under oxidative stress (100-200 mM H2O2) or at low pH (2 and 3). Respiratory activities in A. niger culture show important changes with the osmotic stress increase.

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不同胁迫条件下黑曲霉过氧化氢酶的生产。
在不同的胁迫条件下培养黑曲霉的分生孢子。在6 h的培养基中添加1.2 M NaCl后,过氧化氢酶活性最高,比不添加该抑制剂的对照培养基提高了约2.8倍。在氧化胁迫(100-200 mM H2O2)或低pH(2和3)条件下培养孢子时,过氧化氢酶活性的增加幅度较低(1.3- 1.7倍)。黑曲霉培养的呼吸活性随着渗透胁迫的增加而发生重要变化。
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