{"title":"Mutation in recR gene of Deinococcus radiodurans and possible involvement of its product in the repair of DNA interstrand cross-links","authors":"Shigeru Kitayama , Issay Narumi , Masahiro Kikuchi , Hiroshi Watanabe","doi":"10.1016/S0921-8777(00)00044-6","DOIUrl":null,"url":null,"abstract":"<div><p>We previously reported that some <span><em>Deinococcus radiodurans</em></span> mutants are sensitive to DNA interstrand cross-linking agents but resistant to UV and γ-rays. We isolated DNA fragments from a <em>D. radiodurans</em> genomic library which complemented the mitomycin C sensitivity of one of these mutants. One 3.2<!--> <span>kb-long fragment contains an open reading frame of approximately 700</span> <span>bp and the deduced amino acid sequence<span> is very homologous to other prokaryotic RecR proteins. This open reading frame in the mitomycin C-sensitive mutant strain contains a frame shift mutation at its carboxyl terminal region. These data suggest that RecR protein plays an important role in the resistance to interstrand cross-links in this bacterium.</span></span></p></div>","PeriodicalId":100935,"journal":{"name":"Mutation Research/DNA Repair","volume":"461 3","pages":"Pages 179-187"},"PeriodicalIF":0.0000,"publicationDate":"2000-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0921-8777(00)00044-6","citationCount":"16","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research/DNA Repair","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0921877700000446","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 16
Abstract
We previously reported that some Deinococcus radiodurans mutants are sensitive to DNA interstrand cross-linking agents but resistant to UV and γ-rays. We isolated DNA fragments from a D. radiodurans genomic library which complemented the mitomycin C sensitivity of one of these mutants. One 3.2 kb-long fragment contains an open reading frame of approximately 700bp and the deduced amino acid sequence is very homologous to other prokaryotic RecR proteins. This open reading frame in the mitomycin C-sensitive mutant strain contains a frame shift mutation at its carboxyl terminal region. These data suggest that RecR protein plays an important role in the resistance to interstrand cross-links in this bacterium.
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