{"title":"Purification, characterization and crystallization of enzymes for dibenzothiophene desulfurization.","authors":"T Ohshiro, Y Izumi","doi":"10.1023/a:1008181730720","DOIUrl":null,"url":null,"abstract":"<p><p>DszC and DszA, DBT monooxygenase and DBT sulfone monooxygenase, respectively, involved in dibenzothiophene (DBT) desulfurization, were purified to homogeneity from Rhodococcus erythropolis D-1. The two enzymes were crystallized and enzymologically characterized. We found a high activity of flavin reductase in the non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, which is essential for DszC and A activities, and purified to homogeneity and characterized the enzyme.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 3","pages":"185-8"},"PeriodicalIF":0.0000,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008181730720","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioseparation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1023/a:1008181730720","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15
Abstract
DszC and DszA, DBT monooxygenase and DBT sulfone monooxygenase, respectively, involved in dibenzothiophene (DBT) desulfurization, were purified to homogeneity from Rhodococcus erythropolis D-1. The two enzymes were crystallized and enzymologically characterized. We found a high activity of flavin reductase in the non-DBT-desulfurizing bacterium, Paenibacillus polymyxa A-1, which is essential for DszC and A activities, and purified to homogeneity and characterized the enzyme.
从红红红球菌D-1中纯化出参与二苯并噻吩(DBT)脱硫的DszC和DszA，分别为DBT单加氧酶和DBT砜单加氧酶。对这两种酶进行了结晶和酶学表征。我们在非dbt脱硫菌Paenibacillus polymyxa a -1中发现了高活性的黄素还原酶，该酶对DszC和a的活性至关重要，并对其进行了纯化和表征。
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