Cloning, expression analysis and chromosome mapping of human casein kinase 1 gamma1 (CSNK1G1): identification of two types of cDNA encoding the kinase protein associated with heterologous carboxy-terminal sequences.

J Kusuda, M Hirai, R Tanuma, K Hashimoto
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引用次数: 9

Abstract

Casein kinase 1 gamma1(CK1 gamma1) is known to be involved in the growth and morphogenesis of eukaryotic cells. We have isolated two types of cDNA for human casein kinase 1 gamma1 (hCK1 gamma1). One of them (hCK1 gamma1S) was found to encode a polypeptide consisting of 393 amino acids, which is highly homologous with already reported rat CK1 gamma1 (rCK1 gamma1). The other type of cDNA (hCK1 gamma1L) encodes a polypeptide consisting of 422 amino acids, which is quite identical in the kinase domain, but different in the C-terminal sequence from hCK1 gamma1S. Namely, hCK1 gamma1L has a characteristic sequence of 50 amino acids at the C-terminal end and this motif was shown to be shared by the casein kinase gamma2 and gamma3 from rat and human, suggesting that it is a signature sequence of the gamma-isoforms. In this sense, newly isolated hCK1 gamma1L might be the original form of CK1 gamma1 subspecies rather than rCK1 gamma1 and hCK1 gamma1S. RT-PCR analysis revealed that hCK1 gamma1S mRNA is predominantly present in the testis, whereas the abundance of hCK1 gamma1L mRNA was nearly the same in the twelve tissues examined. These results suggest that novel hCK1 gamma1L may have a unique functional role different from that of hCK1 gamma1S and rCK1 gamma1. The human hCK1 gamma1 gene (CSNK1G1) was mapped to chromosome 15q22.1-->q22.31 by fluorescence in situ hybridization.

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人酪蛋白激酶1 - gamma1 (CSNK1G1)的克隆、表达分析及染色体定位:鉴定与异源羧基末端序列相关的两种激酶蛋白cDNA。
酪蛋白激酶1 γ - 1(CK1 γ - 1)参与真核细胞的生长和形态发生。我们分离了两种人类酪蛋白激酶1 γ - 1 (hCK1 γ - 1)的cDNA。其中一个基因(hCK1 gamma1S)编码一个由393个氨基酸组成的多肽,与已报道的大鼠CK1 gamma1 (rCK1 gamma1)高度同源。另一种类型的cDNA (hCK1 gamma1L)编码一个由422个氨基酸组成的多肽,其激酶结构域与hCK1 gamma1S完全相同,但c端序列与hCK1 gamma1S不同。即,hCK1 γ - 1l在c端具有50个氨基酸的特征序列,该基序被证明与来自大鼠和人类的酪蛋白激酶γ - 2和γ - 3共享,这表明它是γ -同工型的特征序列。从这个意义上说,新分离的hCK1 gammal可能是CK1 gamma1亚种的原始形式,而不是rCK1 gamma1和hCK1 gamma1S。RT-PCR分析显示,hCK1 γ - 1s mRNA主要存在于睾丸中,而hCK1 γ - 1l mRNA的丰度在12个组织中几乎相同。这些结果表明,新的hCK1 gamma1L可能具有不同于hCK1 gamma1S和rCK1 gamma1的独特功能作用。利用荧光原位杂交技术将人类hCK1 γ - 1基因(CSNK1G1)定位于染色体15q22.1—>q22.31。
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