Isolation of alcohol tolerant, osmotolerant and thermotolerant yeast strains and improvement of their alcohol tolerance by UV mutagenesis.

Acta microbiologica Polonica Pub Date : 2002-01-01
Mutlu Nisa Unaldi, Burhan Arikan, Gökhan Coral
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Abstract

In this study, the yeast strains were isolated from grapes by serial dilution technique to determine their alcohol-, sugar- and thermotolerance. 34 wild type yeast strains were isolated and alcohol-, sugar- and thermotolerance of these strains were determined. The maximum alcohol tolerance was found to be 9% (v/v) in yeast strain which is named Y2. Thermotolerance behavior of 6 strains were investigated. The strains were treated with UV light with intervals of 20, 30, 40 and 50 seconds. Selected resistant colonies were investigated for alcohol tolerance. It was found that alcohol tolerance increased from 9% (v/v) to 12% (v/v) on Y2 strain.

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耐酒精、耐渗透和耐热酵母菌株的分离及紫外诱变提高其耐酒精性。
在这项研究中,通过连续稀释技术从葡萄中分离出酵母菌株,以测定其对酒精、糖和热的耐受性。分离了34株野生型酵母菌,测定了这些酵母菌的酒精耐受性、糖耐受性和耐热性。酵母株Y2的最大酒精耐受性为9% (v/v)。对6株菌株的耐热性进行了研究。分别以20、30、40和50秒的时间间隔进行紫外处理。对选定的抗性菌落进行酒精耐受性研究。结果表明,Y2菌株的酒精耐受性由9% (v/v)提高到12% (v/v)。
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