Herpes Simplex Type I Virus Infection of Cultured Human Vascular Endothelial Cells: Expression of Cell Adhesion Molecules and Induction of Interferon and Cytokine Production by Blood Mononuclear Cells.

Abstract

The expression of cell adhesion molecules, P- and E-selectins, ICAM-1, and VCAM-1, was studied in cultured human vascular endothelial cells (ECs) infected by herpes simplex type I virus (HSV-1). It was shown that ECs without any signs of the cytopathogenic effect (CPE) expressed on their surface P- and E-selectins as soon as 24 h after infection. No appearance of VCAM-1 or increase in ICAM-1 expression was detected. Peripheral blood mononuclear cells (PBMCs) isolated by gradient centrifugation adhered preferentially with HSV-1-infected morphologically unchanged ECs but not with cells modified in result of CPE. The interferon and cytokine production by PBMCs was assayed after their contact with infected and glutaraldehyde-fixed ECs. The secretion of IFN-alpha, IFN-gamma, IL-1, IL-6, and TNF-alpha (but not of IL-4) was found to be inducible and correlated with the multiplicity of infection. Obtained results allow to consider a described cell culture system as a model for further investigation of initial stages of HSV-1 infection and of pathogenesis of vascular disease.