[Direct visualization of microcirculation in burn wounds with OPS imaging--is determination of depth of burns possible?].

S Langer, R Hatz, K Messmer, H H Homann, F W Peter, H U Steinau
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Abstract

Adjunct diagnostic techniques might help surgeons to accurately analyse the depth of a burn. However, despite all technical innovations an ideal device for such an application has not been established for routine use as yet. OPS imaging implemented into the CYTOSCAN A/R is a new, recently introduced technique which allows to obtain high contrast images of the microcirculation without the necessity for fluorescent dyes. The aim of the study was to validate OPS imaging as a tool to study microcirculation in skin after burn injury. OPS imaging was applied by no-touch technique and capillary blood flow was videotaped. Subsequent measurements of the microcirculation were performed at the identical site of the burn. Quantitative analysis of the microcirculation was performed off-line using CapImage. OPS imaging produces high quality images of the microcirculation in a burn wound. Data is given as the number of perfused capillaries per observation area (functional capillary density; FCD) [n/cm2]. OPS imaging allows for direct in vivo visualization and quantification of the microcirculation in burned skin. Our preliminary results of the use of OPS imaging in assessing the microcirculation in burns appear promising, and we hope that this novel technique will allow to improve the knowledge of the dynamics of the microcirculation in the pathophysiology of thermal injury.

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[使用OPS成像直接显示烧伤创面的微循环——是否可能确定烧伤深度?]。
辅助诊断技术可以帮助外科医生准确分析烧伤的深度。然而,尽管所有的技术创新,这种应用的理想装置尚未建立常规使用。在CYTOSCAN A/R中实现的OPS成像是最近引入的一种新技术,它允许在不需要荧光染料的情况下获得微循环的高对比度图像。本研究的目的是验证OPS成像作为研究烧伤后皮肤微循环的工具。采用非接触技术进行OPS成像,并对毛细血管血流进行录像。随后的微循环测量在烧伤的同一部位进行。离线使用CapImage进行微循环定量分析。OPS成像产生烧伤创面微循环的高质量图像。数据为每个观察区域的灌注毛细血管数(功能毛细血管密度;FCD) (n / cm2)。OPS成像允许对烧伤皮肤的微循环进行直接的体内可视化和量化。我们使用OPS成像评估烧伤微循环的初步结果看起来很有希望,我们希望这项新技术能够提高热损伤病理生理学中微循环动力学的知识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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