Suppression of BCR-ABL mRNA by various ribozymes in HeLa cells.

Y Kato, T Kuwabara, H Toda, M Warashina, K Taira
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引用次数: 5

Abstract

Ribozymes are RNA molecules with enzymatic activity that can cleave target RNA molecules in a sequence specific manner. To date, various types of ribozyme have been constructed to cleave other RNAs and such trans-acting ribozymes include hammerhead, hairpin and HDV ribozymes. External guide sequence (EGS) can also induce the suppression of a gene-expression by taking advantage of cellular RNase P. Here we compared the activities of various functional RNA cleavers both in vitro and in vivo. The first purpose of this comparison was intended to determine the best ribozyme motif with the highest activity in cells. The second purpose is to know the correlation between the activities of ribozymes in vitro and in vivo. Our results indicated that the intrinsic cleavage activity of ribozymes is not the sole determinant that is responsible for the activity of a ribozyme in cultured cells.

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不同核酶对HeLa细胞BCR-ABL mRNA的抑制作用。
核酶是一种具有酶活性的RNA分子,能够以特定的序列方式切割目标RNA分子。迄今为止,已经构建了各种类型的核酶来切割其他rna,这些反式核酶包括锤头核酶、发夹核酶和HDV核酶。外部引导序列(EGS)也可以利用细胞RNase p诱导基因表达的抑制。在这里,我们比较了各种功能性RNA切割器在体外和体内的活性。这个比较的第一个目的是为了确定细胞中活性最高的最佳核酶基序。第二个目的是了解核酶在体内和体外的活性之间的相关性。我们的结果表明,核酶的内在裂解活性不是唯一的决定因素,负责核酶的活性在培养细胞。
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