Genome structure of Ri plasmid (3). Sequencing analysis of the vir region of pRi1724 in Japanese Agrobacterium rhizogenes.

N Satuti, K Moriguchi, M Sato, M Kataoka, Y Maeda, N Tanaka, K Yoshida
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引用次数: 4

Abstract

The entire genome of the pRi1724 (217.6-kb) in the mikimopine type Agrobacterium rhizogenes strain MAFF03-01724 has been completely sequenced. The vir region covering 30.2-kb has found to be composed of 21 genes resembling virH1, virA, virB1-11, virG, virC1-2, and virD1-5. The structural organization of the pRi1724 vir operons in this study is exactly the same as that of the previously reported vir operons of other Ri or Ti plasmids, although the size of some ORFs showed little variations among the plasmids. We also found virE3 gene in the pRi1724 (1), but different from Ti plasmids, virE1 and virE2 that are also important for the virulence do not exist in the vir region of pRi1724.

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Ri质粒的基因组结构(3).日本根农杆菌pRi1724 vir区序列分析。
mikimopine型根际农杆菌MAFF03-01724的pRi1724全基因组(217.6 kb)已被完全测序。vir区覆盖30.2 kb,由21个类似virH1、virA、virB1-11、virG、virC1-2和virD1-5的基因组成。本研究中pRi1724的vir操纵子的结构组织与之前报道的其他Ri或Ti质粒的vir操纵子完全相同,尽管一些orf的大小在质粒之间几乎没有变化。我们在pRi1724中也发现了virE3基因(1),但与Ti质粒不同的是,在pRi1724的vir区不存在对毒力同样重要的virE1和virE2。
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