Mechanism of garlic (Allium sativum) induced reduction of hypertension in 2K-1C rats: a possible mediation of Na/H exchanger isoform-1.

IF 2.9 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 2003-10-01 DOI:10.1016/s0952-3278(03)00087-5

K K Al-Qattan, I Khan, M A Alnaqeeb, M Ali

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引用次数: 55

Abstract

Garlic causes reduction in blood pressure (BP), however the role of Na/H exchanger (NHE) which mediates hypertension and related tissue-damage is poorly understood. In this study the effect of an established dose of raw garlic extract was investigated on the expression of NHE-1 and -3 and sodium pump activity in a 2K-1C model of hypertension in rats. 2K-1C animals showed high BP, increased serum concentration of PGE2 and TxB2, hypertrophy of the unclipped kidneys, but not in the clipped kidneys In addition, NHE-1 and NHE-3 isoforms were increased in both the 2K-1C kidneys, whereas alpha-actin was increased in the clipped but not in unclipped kidneys. Sodium pump activity was decreased in the clipped kidneys, but remained unchanged in the unclipped kidneys. Garlic treatment reduced the induction of NHE-1 only in the unclipped 2K-1C kidneys, whereas garlic treatment increased the sodium pump activity in both the 2K-1C kidneys. These findings demonstrate that the antihypertensive action of garlic is associated with a reversal of NHE-1 induction in the unclipped kidneys. Induction of NHE isoforms together with a reduced sodium pump activity might cause necrosis in the 2K-1C clipped kidneys due to cellular retention of Na+. On the other hand, activation of sodium pump by garlic extract in the kidneys should reduce intracellular Na+ concentration and normalize BP. These findings signify the use of garlic in the treatment of hypertension.

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大蒜(Allium sativum)降低2K-1C大鼠高血压的机制:Na/H交换物异构体-1的可能介导

大蒜可以降低血压(BP)，但Na/H交换剂(NHE)介导高血压和相关组织损伤的作用尚不清楚。本研究探讨了一定剂量生大蒜提取物对高血压大鼠2K-1C模型中NHE-1和-3表达及钠泵活性的影响。2K-1C大鼠血压升高，血清PGE2和TxB2浓度升高，未切除的肾脏肥大，而切除的肾脏没有。此外，在2K-1C大鼠的肾脏中，NHE-1和NHE-3异构体均升高，而在切除的肾脏中，α -肌动蛋白升高，而在未切除的肾脏中则没有。钠泵活性在切除肾脏中降低，但在未切除肾脏中保持不变。大蒜处理仅在未剪断的2K-1C肾脏中减少了NHE-1的诱导，而大蒜处理增加了2K-1C肾脏中的钠泵活性。这些发现表明，大蒜的降压作用与未切除肾脏中NHE-1诱导的逆转有关。NHE异构体的诱导和钠泵活性的降低可能由于Na+的细胞滞留而导致2K-1C切除肾的坏死。另一方面，大蒜提取物激活肾脏钠泵可降低细胞内Na+浓度，使血压恢复正常。这些发现表明大蒜可用于治疗高血压。

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来源期刊

Prostaglandins, leukotrienes, and essential fatty acids 医学-内分泌学与代谢

CiteScore

6.40

自引率

6.70%

发文量

审稿时长

13.2 weeks

期刊介绍： The role of lipids, including essential fatty acids and their prostaglandin, leukotriene and other derivatives, is now evident in almost all areas of biomedical science. Cell membrane behaviour and cell signalling in all tissues are highly dependent on the lipid constituents of cells. Prostaglandins, Leukotrienes & Essential Fatty Acids aims to cover all aspects of the roles of lipids in cellular, organ and whole organism function, and places a particular emphasis on human studies. Papers concerning all medical specialties are published. Much of the material is particularly relevant to the development of novel treatments for disease.