Proteomic Changes in Methicillin-Resistant Staphylococcus aureus Exposed to Cannabinoids

IF 3.3 2区生物学 Q2 CHEMISTRY, MEDICINAL Journal of Natural Products Pub Date : 2023-07-06 DOI:10.1021/acs.jnatprod.3c00064

Jan Struckmann Poulsen, Christina Kjærager Nielsen, Nina Ahrendt Pedersen, Reinhard Wimmer, Teis Esben Sondergaard, Nadieh de Jonge and Jeppe Lund Nielsen*,

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a major human pathogen that causes a wide range of infections. Its resistance to β-lactam antibiotics complicates treatment due to the limited number of antibiotics with activity against MRSA. To investigate development of alternative therapeutics, the mechanisms that mediate antibiotic resistance in MRSA need to be fully understood. In this study, MRSA cells were subjected to antibiotic stress from methicillin in combination with three cannabinoid compounds and analyzed using proteomics to assess the changes in physiology. Subjecting MRSA to nonlethal levels of methicillin resulted in an increased production of penicillin-binding protein 2 (PBP2). Exposure to cannabinoids showed antibiotic activity against MRSA, and differential proteomics revealed reduced levels of proteins involved in the energy production as well as PBP2 when used in combination with methicillin.

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暴露于大麻素的耐甲氧西林金黄色葡萄球菌的蛋白质组学变化

耐甲氧西林金黄色葡萄球菌(MRSA)是一种主要的人类病原体，引起广泛的感染。由于具有抗MRSA活性的抗生素数量有限，其对β-内酰胺类抗生素的耐药性使治疗复杂化。为了研究替代疗法的发展，需要充分了解MRSA介导抗生素耐药性的机制。在这项研究中，MRSA细胞受到甲氧西林联合三种大麻素化合物的抗生素应激，并使用蛋白质组学分析来评估生理变化。将MRSA置于非致死水平的甲氧西林导致青霉素结合蛋白2 (PBP2)的产生增加。暴露于大麻素中显示出对MRSA的抗生素活性，差异蛋白质组学显示，当与甲氧西林联合使用时，参与能量产生的蛋白质和PBP2水平降低。

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来源期刊

Journal of Natural Products 生物-药学

CiteScore

9.10

自引率

5.90%

发文量

294

审稿时长

2.3 months

期刊介绍： The Journal of Natural Products invites and publishes papers that make substantial and scholarly contributions to the area of natural products research. Contributions may relate to the chemistry and/or biochemistry of naturally occurring compounds or the biology of living systems from which they are obtained. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin. When new compounds are reported, manuscripts describing their biological activity are much preferred. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin.