Morphology of isolated rabbit heart muscle mitochondria and the oxidation of extramitochondrial reduced diphosphopyridine nucleotide.

P D DESHPANDE, D D HICKMAN, R W VON KORFF
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引用次数: 20

Abstract

The morphology of rabbit heart muscle mitochondria isolated in several media has been compared by electron microscopy. The internal structure of isolated mitochondria differs from that of in situ mitochondria, with the type and degree of alteration depending on the isolation medium. Examination of the isolated mitochondria after incubation revealed that additional morphological changes occurred during incubation, but these changes were less pronounced when the incubation was conducted in a complete medium containing substrate. The isolated mitochondria have been shown to be capable of catalyzing a slow aerobic oxidation of extramitochondrial reduced diphosphopyridine nucleotide. The rate of DPNH oxidation observed is sufficient to account for the ability of the mitochondria to oxidize lactate in the presence of catalytic amounts of DPNH. The suspensions used were essentially free of mitochondrial fragments, which are known to oxidize DPNH. Possible relationships of these findings to metabolism in situ are discussed. The results indicate the desirability of correlating biochemical activities with the morphology of isolated mitochondria.

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离体兔心肌线粒体形态及线粒体外还原二磷酸吡啶核苷酸的氧化。
用电镜对几种培养基分离的兔心肌线粒体的形态进行了比较。分离线粒体的内部结构不同于原位线粒体,其类型和程度取决于分离介质。孵育后对分离线粒体的检查显示,在孵育期间发生了额外的形态学变化,但当孵育在含有底物的完整培养基中进行时,这些变化不太明显。分离的线粒体已被证明能够催化线粒体外还原二磷酸吡啶核苷酸的缓慢有氧氧化。观察到的DPNH氧化速率足以说明线粒体在催化量的DPNH存在下氧化乳酸的能力。所使用的悬浮液基本上不含线粒体片段,已知线粒体片段会氧化DPNH。讨论了这些发现与原位代谢的可能关系。结果表明,将生物化学活性与分离线粒体的形态联系起来是可取的。
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