Metabolism in vivo of steroids by the canine testes

Kristen B. Eik-nes, Madhukar Kekre
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引用次数: 36

Abstract

The formation of testosterone in vivo has been investigated in anesthetized dogs by infusing radioactive steroids via the left spermatic artery and isolating radioactive testosterone in venous blood obtained from the left spermatic vein. The data from such experiments indicate that the formation of 17α-hydroxypregnenolone and the metabolism of this steroid to testosterone is a major route in the biosynthesis in vivo of testosterone from pregnenolone by the canine testis.

  • 1.

    1. When an equal amount of [4−14C]progesterone and 17α-hydroxy[7α-3H]pregnenolone were infused at a constant rate by the spermatic artery, the concentration of 3H in isolated spermatic vein testosterone was from 3–8 times that of 14C.

  • 2.

    2. When [7α-3H]prenenolone was infused by the left spermatic artery for 5 min, more [3H]17α-hydroxypregnenolone than [3H]progesterone was found in the left testis at the end of this infusion.

  • 3.

    3. The problem of preferred metabolism to testosterone in vivo by testicular tissue is discussed.

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犬睾丸体内类固醇的代谢
通过左精索动脉注入放射性类固醇,并从左精索静脉静脉血中分离放射性睾酮,研究了麻醉犬体内睾酮的形成。这些实验数据表明,17α-羟基孕烯醇酮的形成并将其代谢为睾酮是犬睾丸体内孕烯醇酮生物合成睾酮的主要途径。1.1. 当精索动脉等量灌注[4−14C]孕酮和17α-羟基[7α-3H]孕烯醇酮时,离体精索静脉3H睾酮浓度为14C的3-8倍。左精索动脉灌注[7α-3H]苯烯诺酮5 min后,灌注结束时左睾丸内[3H]17α-羟孕烯诺酮含量高于[3H]孕酮含量。讨论了睾丸组织在体内对睾酮的优先代谢问题。
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