Réaction phosphoroclastique du pyruvate par Clostridium saccharobutyricum Mise en évidence de l'intervention d'un cofacteur de nature ptéridique dans la dégradation du pyruvate
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引用次数: 1
Abstract
Clostridium saccharobutyricum extracts, heat treated at 65° have somewhat similar properties to iron-deficient extracts prepared from this organism: they cannot degrade pyruvate by the phosphoroclastic pathway or incorporate 14CO2 into pyruvate. However, they stimulate the clastic activity of normal extracts and restore the clastic activity of aged extracts. A mixture of heat-treated (65°) and iron-deficient extracts is inactive. The heat-treated (65°) extract has no stimulating effect on the 14CO2-pyruvate exchange reaction. A cofactor FCL, of the formylfolic acid type was isolated from at 65° treated extracts. It increases the rat off oxidation of pyruvate. N10-formyltetrahydrofolic acid replaces FCL whereas folic, N10-formylfolic and N5-formyltetrahydrofolic acids are inactive.
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