A deoxyribonucleic acid-protein complex having DNA-polymerase and RNA-polymerase activities in cell-free extracts of Escherichia coli

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI:10.1016/0926-6550(64)90168-9

Motoko Kadoya, Hiromi Mitsui , Yasuyuki Takagi , Eiko Otaka , Hideho Suzuki , Syozo Osawa

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引用次数: 52

Abstract

1.
1. A deoxyribonucleoprotein, found in extracts of Escherichia coli B(H), has been investigated with respect to its role in DNA and RNA synthesis.
2.
2. A deoxyribonucleoprotein in ribosome-free cell extract could be separated from the other cellular components either with gel filtration or zone electrophoresis.
3.
3. The isolated deoxyribonucleoprotein was composed of roughly 80% of DNA a and 20% of protein.
4.
4. Association of DNA polymerase (deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7) and RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase (DNA dependent), EC 2.7.7.6) with this deoxyribonucleoprotein has been demonstrated.
5.
5. DNA polymerase and RNA polymerase, purified from E. coli extract, had an ability to form a complex with DNA.

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在大肠杆菌的无细胞提取物中具有dna聚合酶和rna聚合酶活性的脱氧核糖核酸-蛋白质复合物

1.1. 在大肠杆菌B(H)提取物中发现的一种脱氧核糖核蛋白，已被研究其在DNA和RNA合成中的作用。无核糖体细胞提取物中的脱氧核糖核蛋白可以用凝胶过滤或带电泳从其他细胞组分中分离出来。分离的脱氧核糖核蛋白由大约80%的DNA a和20%的蛋白质组成。DNA聚合酶(脱氧核苷三磷酸:DNA脱氧核苷酸转移酶，EC 2.7.7.7)和RNA聚合酶(核苷三磷酸:RNA核苷酸转移酶(DNA依赖)，EC 2.7.7.6)与这种脱氧核糖核蛋白的关联已被证实。从大肠杆菌提取物中纯化的DNA聚合酶和RNA聚合酶具有与DNA形成复合物的能力。

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects

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Author index Erratum Subject index Changes in sedimentation properties of ribosomal ribonucleic acids during the course of ribosome formation in Escherichia coli The inhibition of deoxyribonucleotidyl transferase, DNAase and RNAase by sodium poly ethenesulfonic acid. Effect of the molecular weight of the inhibitor