{"title":"The inhibition of DNA polymerase by extracts of rat liver partial purification and properties of a deoxyribonuclease","authors":"Margery G. Burdon, R.M.S. Smellie, J.N. Davidson","doi":"10.1016/0926-6550(64)90169-0","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The inhibitory effect of extracts of rat liver on DNA polymerase (deoxyribonucleosidetriphosphate: DNA deoxyribonucleotidyltransferase, EC 2.7.7.7) has been investigated.</p></span></li><li><span>2.</span><span><p>2. Fractionation of the crude extracts has revealed that the inhibition is not due to the hydrolysis of the deoxyribonucleoside 5′-triphosphates by phosphatases.</p></span></li><li><span>3.</span><span><p>3. The inhibitory factor was purified about 10–15-fold, with a yield of 20%.</p></span></li><li><span>4.</span><span><p>4. Its activity was due to the action on the primer DNA of a deoxyribonuclease, and the oligonucleotide products of the reaction inhibited the polymerase.</p></span></li><li><span>5.</span><span><p>5. The properties of the deoxyribonuclease differed in some respects from those of splenic deoxyribonuclease II (deoxyribonucleate 3′-nucleotidohydrolase, EC 3.1.4.6) and pancreatic deoxyribonuclease I (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.5). In particular, it hydrolyses thermally denatured DNA more rapidly than native DNA.</p></span></li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 46-58"},"PeriodicalIF":0.0000,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90169-0","citationCount":"12","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064901690","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 12
Abstract
1.
1. The inhibitory effect of extracts of rat liver on DNA polymerase (deoxyribonucleosidetriphosphate: DNA deoxyribonucleotidyltransferase, EC 2.7.7.7) has been investigated.
2.
2. Fractionation of the crude extracts has revealed that the inhibition is not due to the hydrolysis of the deoxyribonucleoside 5′-triphosphates by phosphatases.
3.
3. The inhibitory factor was purified about 10–15-fold, with a yield of 20%.
4.
4. Its activity was due to the action on the primer DNA of a deoxyribonuclease, and the oligonucleotide products of the reaction inhibited the polymerase.
5.
5. The properties of the deoxyribonuclease differed in some respects from those of splenic deoxyribonuclease II (deoxyribonucleate 3′-nucleotidohydrolase, EC 3.1.4.6) and pancreatic deoxyribonuclease I (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.5). In particular, it hydrolyses thermally denatured DNA more rapidly than native DNA.
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