Studies on the nucleotide arrangement in deoxyribonucleic acids VIII. A comparison of procedures for the determination of the frequency of pyrimidine nucleotide runs

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI:10.1016/0926-6550(64)90250-6

Herman S. Shapiro, Erwin Chargaff

{"title":"Studies on the nucleotide arrangement in deoxyribonucleic acids VIII. A comparison of procedures for the determination of the frequency of pyrimidine nucleotide runs","authors":"Herman S. Shapiro, Erwin Chargaff","doi":"10.1016/0926-6550(64)90250-6","DOIUrl":null,"url":null,"abstract":"<div><p>Two DNA preparations of mammalian origin, <em>viz</em>., the DNA from calf thymus and the DNA from human spleen, were employed in a comparative study of different methods suitable for the investigation of the arrangement of pyrimidine nucleotides in the polymer chain. The conditions under comparison were (a) the degradation of DNA with dilute sulfuric acid; (b) the degradation of DNA with formic acid-diphenylamine; (c) the degradation of apurinic acid with dilute alkali. The released pyrimidine nucleotide runs of length 1 to 6 were determined directly by two-dimensional paper chromatography.</p><p>The range and validity of the several procedures described here and in the literature are considered in detail and the bearing of the findings on the general problem of the nucleotide sequence in DNA is discussed.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 2","pages":"Pages 262-270"},"PeriodicalIF":0.0000,"publicationDate":"1964-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90250-6","citationCount":"54","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064902506","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 54

Abstract

Two DNA preparations of mammalian origin, viz., the DNA from calf thymus and the DNA from human spleen, were employed in a comparative study of different methods suitable for the investigation of the arrangement of pyrimidine nucleotides in the polymer chain. The conditions under comparison were (a) the degradation of DNA with dilute sulfuric acid; (b) the degradation of DNA with formic acid-diphenylamine; (c) the degradation of apurinic acid with dilute alkali. The released pyrimidine nucleotide runs of length 1 to 6 were determined directly by two-dimensional paper chromatography.

The range and validity of the several procedures described here and in the literature are considered in detail and the bearing of the findings on the general problem of the nucleotide sequence in DNA is discussed.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

脱氧核糖核酸中核苷酸排列的研究。嘧啶核苷酸运行频率测定方法的比较

本文采用犊牛胸腺DNA和人脾脏DNA两种哺乳动物来源的DNA制备方法，比较研究了适于研究聚合物链中嘧啶核苷酸排列的不同方法。比较的条件是:(a)稀硫酸对DNA的降解;(b)甲酸-二苯胺对DNA的降解;(c)稀碱对无尿酸的降解。释放的长度为1 ~ 6的嘧啶核苷酸序列用二维纸层析直接测定。这里和文献中描述的几个程序的范围和有效性被详细考虑，并讨论了对DNA中核苷酸序列的一般问题的发现的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects

自引率

0.00%

发文量

期刊最新文献

Author index Erratum Subject index Changes in sedimentation properties of ribosomal ribonucleic acids during the course of ribosome formation in Escherichia coli The inhibition of deoxyribonucleotidyl transferase, DNAase and RNAase by sodium poly ethenesulfonic acid. Effect of the molecular weight of the inhibitor