Purification and chemical characterization of alanine dehydrogenase of Bacillus subtilis

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-10-23 DOI:10.1016/0926-6569(64)90266-4

Akira Yoshida, Ernst Freese

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引用次数: 60

Abstract

1.
1. Bacillus subtilis alanine dehydrogenase (L-alanine: NAD oxidoreductase (deaminating), EC_1.4.1.1. has been purified by column chromatography using calcium phosphate gel, DEAE-Sephadex and Ecteola-cellulose; it has been obtained in crystalline form.
2.
2. The sedimentation pattern indicated a homogenous preparation. The sedimentation constant at infinite dilution was 8.8 S. The molecular weight estimated by the sedimentation equilibrium method was 228 000.
3.
3. Amino acid analysis gave the following ratios of amino acid residues: Asp, ₄₃; Thr, ₃₄; Ser, ₂₀; Glu, ₅₁; Pro, ₂₅; Gly, ₅₁; Ala, ₆₅; CySH, ₂; Val, ₄₉; Met, ₁₁; iLeu, ₂₈; Leu, ₄₂; Tyr, ₁₅; Phe, ₇; Try, ₁; Lys, ₂₈; His, ₉; Arg, ₁₄.

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枯草芽孢杆菌丙氨酸脱氢酶的纯化及化学性质研究

1.1. 枯草芽孢杆菌丙氨酸脱氢酶(l -丙氨酸:NAD氧化还原酶(脱氨)，EC1.4.1.1。经磷酸钙凝胶、DEAE-Sephadex和Ecteola-cellulose柱层析纯化;它以结晶形式得到。沉积模式表明是一种均质制剂。无限稀释下的沉淀常数为8.8 s，沉淀平衡法估算的分子量为228 000.3.3。氨基酸分析得到氨基酸残基比值:Asp, 43;34岁的刺;Ser 20;Glu, 51;专业,25岁;通用电气,51;阿拉巴马州,65;CySH 2;瓦尔,49个;满足,11;iLeu, 28日;低浓缩铀,42岁;酪氨酸,15;板式换热器,7;试,1;赖氨酸,28日;他,9;参数,14。

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects

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