{"title":"Light-induced rapid absorption changes during photosynthesis","authors":"Bacon Ke","doi":"10.1016/0926-6577(64)90185-8","DOIUrl":null,"url":null,"abstract":"<div><p>Fresh chloroplasts treated with appropriate amounts of digitonin give light-induced absorption changes in the Soret-band and far-red regions. The absorption changes are enhanced by ascorbate and eliminated by ferricyanide. The light-minus-dark difference spectrum of digitonin-treated chloroplasts agrees closely with that of the pigment complex P 700 reported by <span>Kok</span>. Dichlorophenolindophenol does not appear to mediate in the electron transfer between ascorbate and the photooxidized chlorophyll complex, whereas phenazine methosulfate, depending on its concentration, greately modifies the kinetics of the re-duction of the pigment complex by ascorbate.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1964-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90185-8","citationCount":"16","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926657764901858","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 16
Abstract
Fresh chloroplasts treated with appropriate amounts of digitonin give light-induced absorption changes in the Soret-band and far-red regions. The absorption changes are enhanced by ascorbate and eliminated by ferricyanide. The light-minus-dark difference spectrum of digitonin-treated chloroplasts agrees closely with that of the pigment complex P 700 reported by Kok. Dichlorophenolindophenol does not appear to mediate in the electron transfer between ascorbate and the photooxidized chlorophyll complex, whereas phenazine methosulfate, depending on its concentration, greately modifies the kinetics of the re-duction of the pigment complex by ascorbate.
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