{"title":"Pseudomonas aeruginosa peptide peptidohydrolase IV. Optical rotatory dispersion and amino acid composition","authors":"Kazuyuki Morihara, Nobuo Yoshida, Kaoru Kuriyama","doi":"10.1016/0926-6569(64)90194-4","DOIUrl":null,"url":null,"abstract":"<div><p>Some physico-chemical properties of the crystalline proteinase (peptide peptidohydrolase) from <em>Pseudomonas aeruginosa</em> IFO 3080 were studied. The [α]<sub>D</sub> was −30°. The λ<sub>c</sub> was 227.6 mμ and <em>b</em><sub>0</sub> was −30, derived from the optical rotatory dispersion. The helical content was calculated as 5.2% from the value of <em>b</em><sub>0</sub>. The amino acid composition of the proteinase was as follows: aspartic acid 66, threonine 24, serine 42, glutamic acid 35, proline 11, glycine 65, alanine 58, valine 25, isoleucine 17, leucine 37, tyrosine 21, phenylalanine 20, lysine 16, histidine 6, arginine 7, tryptophan 6. Amino acids containing sulfur, such as cystine, cysteine and methionine, were not detected.</p></div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 2","pages":"Pages 361-366"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90194-4","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926656964901944","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 11
Abstract
Some physico-chemical properties of the crystalline proteinase (peptide peptidohydrolase) from Pseudomonas aeruginosa IFO 3080 were studied. The [α]D was −30°. The λc was 227.6 mμ and b0 was −30, derived from the optical rotatory dispersion. The helical content was calculated as 5.2% from the value of b0. The amino acid composition of the proteinase was as follows: aspartic acid 66, threonine 24, serine 42, glutamic acid 35, proline 11, glycine 65, alanine 58, valine 25, isoleucine 17, leucine 37, tyrosine 21, phenylalanine 20, lysine 16, histidine 6, arginine 7, tryptophan 6. Amino acids containing sulfur, such as cystine, cysteine and methionine, were not detected.
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