{"title":"Effect of thiols on extraction and activity of ox-kidney urate oxidase","authors":"R. Truscoe","doi":"10.1016/0926-6569(64)90185-3","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The yield of urate oxidase (urate:O<sub>2</sub> oxidoreductase, EC 1.7.3.3) extracted from ox-kidney acetone powder by 0.2 M Tris (pH 10.5), was raised 1.5- to 10-fold by addition of a number of thiols, at optimum concentrations of about 8 mM.</p></span></li><li><span>2.</span><span><p>2. These thiols fell into three groups, according to their action on urate oxidase extracts: (i) thioglycollic acid, BAL, mercaptoethanol, penicillamine, and ethanedithiol: (i) thioglycollic acid, BAL, mercaptoethanol, penicillamine, and etahnedithiol: no effect on urate oxidase activity; (ii) cysteine: increasing inhibition with rising concentration, up to 32 mM; (iii) GSH: increasing potentiation up to 8 mM; GSSG had no effect.</p></span></li></ul></div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 2","pages":"Pages 278-285"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90185-3","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926656964901853","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
1.
1. The yield of urate oxidase (urate:O2 oxidoreductase, EC 1.7.3.3) extracted from ox-kidney acetone powder by 0.2 M Tris (pH 10.5), was raised 1.5- to 10-fold by addition of a number of thiols, at optimum concentrations of about 8 mM.
2.
2. These thiols fell into three groups, according to their action on urate oxidase extracts: (i) thioglycollic acid, BAL, mercaptoethanol, penicillamine, and ethanedithiol: (i) thioglycollic acid, BAL, mercaptoethanol, penicillamine, and etahnedithiol: no effect on urate oxidase activity; (ii) cysteine: increasing inhibition with rising concentration, up to 32 mM; (iii) GSH: increasing potentiation up to 8 mM; GSSG had no effect.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
