Studies on animal hemoglobins II. The influence of inorganic phosphate on the physico-chemical and physiological properties of the hemoglobin of the adult chicken
T.H.J. Huisman, J.M.Schillhorn Van Veen, A.M. Dozy, C.M. Nechtman
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引用次数: 20
Abstract
The equilibrium of dilute and concentrated adult chicken red blood cell hemolysates with O2 has been studied using potassium phosphate buffers and NaCl solutions of different molarities. The results indicate an increase in O2 affinity with increase in ionic strength. The same phenomenon was observed for the isolated minor hemoglobin component; the isolated major hemoglobin component showed a rather high O2 affinity at low ionic strength, which decreased with increase in molarity. The minor hemoglobin fraction exhibited an ability to form rather stable complexes with phosphate ions; such complexes possessed a high O2 affinity at low ionic strength. The data also indicated that the two hemoglobin components in a hemolysate interacted with each other. This extra-molecular interaction interfered with the physiologic properties of the hemolysate. No physico-chemical interaction could be detected, as was demonstrated by the determination of the molecular weight and by viscosity measurements.