{"title":"Purification of protamine sulfate for the quantitative precipitation of ribonucleic acids","authors":"Georg R. Philipps, Jane West","doi":"10.1016/0926-6550(64)90071-4","DOIUrl":null,"url":null,"abstract":"<div><p>A method is described for the purification of protamine sulfate by chromatography on DEAE-cellulose. The precipitation of soluble RNA and ribosomal RNA by purified protamine is quantitative, while oligonucleotides of low molecular weight are not precipitated. The procedure allows the quantitative removal of soluble RNA from solutions and the preparation of soluble RNA samples of high purity.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 3","pages":"Pages 416-420"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90071-4","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064900714","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
A method is described for the purification of protamine sulfate by chromatography on DEAE-cellulose. The precipitation of soluble RNA and ribosomal RNA by purified protamine is quantitative, while oligonucleotides of low molecular weight are not precipitated. The procedure allows the quantitative removal of soluble RNA from solutions and the preparation of soluble RNA samples of high purity.
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