{"title":"Nuclease-resistant sequences in ultraviolet-irradiated deoxyribonucleic acid","authors":"R.B. Setlow, W.L. Carrier, F.J. Bollum","doi":"10.1016/0926-6550(64)90075-1","DOIUrl":null,"url":null,"abstract":"<div><p>Enzymic hydrolysis of ultraviolet-irradiated DNA with venom phosphodiesterase (EC 3.1.4.1) yields oligonucleotide sequences that are resistant to enzymic degradation. Most of the oligonucleotides contain thymine dimers in sequences whose general structure is pXp<span><math><mtext>TpT</mtext></math></span>. The number and type of the dimer-containing trinucleotides depend on the base composition of the DNA, the irradiating wavelength, and the incident dose. Dimers in native DNA are eliminated by treatment of the DNA with an extract from yeast in the presence of 360-mμ light. They are eliminated very slowly from denatured DNA and not at all from trinucleotides. The splitting of dimers in trinucleotides by 240-mμ irradiation is independent of the base X in pXp<span><math><mtext>TpT</mtext></math></span>, but the rate of formation of dimers by 280 mμ depends on the third base.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 3","pages":"Pages 446-461"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90075-1","citationCount":"66","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064900751","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 66
Abstract
Enzymic hydrolysis of ultraviolet-irradiated DNA with venom phosphodiesterase (EC 3.1.4.1) yields oligonucleotide sequences that are resistant to enzymic degradation. Most of the oligonucleotides contain thymine dimers in sequences whose general structure is pXp. The number and type of the dimer-containing trinucleotides depend on the base composition of the DNA, the irradiating wavelength, and the incident dose. Dimers in native DNA are eliminated by treatment of the DNA with an extract from yeast in the presence of 360-mμ light. They are eliminated very slowly from denatured DNA and not at all from trinucleotides. The splitting of dimers in trinucleotides by 240-mμ irradiation is independent of the base X in pXp, but the rate of formation of dimers by 280 mμ depends on the third base.
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