{"title":"Pyruvate — Oxaloacetate exchange reaction in baker's yeast","authors":"Jura Gailiusis, Robert W. Rinne, C.R. Benedict","doi":"10.1016/0926-6569(64)90019-7","DOIUrl":null,"url":null,"abstract":"<div><p>The results in this paper show that extracts of baker's yeast contain a pyruvate carboxylase which catalyzes the carboxylation of pyruvate to form oxaloacetate and the exchange of oxaloacetate and pyruvate. The carboxylation reaction requires GSH, MgCl<sub>2</sub>, ATP and is stimulated by acetyl-CoA. In the exchange reaction the transfer of the β-COOH group of oxaloacetate to pyruvate does not require ATP and is not stimulated by acetyl-CoA. Both reactions are inhibited by low concentrations of avidin and <em>p</em>-chloromercuribenzoate.</p></div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 3","pages":"Pages 595-601"},"PeriodicalIF":0.0000,"publicationDate":"1964-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90019-7","citationCount":"38","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926656964900197","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 38
Abstract
The results in this paper show that extracts of baker's yeast contain a pyruvate carboxylase which catalyzes the carboxylation of pyruvate to form oxaloacetate and the exchange of oxaloacetate and pyruvate. The carboxylation reaction requires GSH, MgCl2, ATP and is stimulated by acetyl-CoA. In the exchange reaction the transfer of the β-COOH group of oxaloacetate to pyruvate does not require ATP and is not stimulated by acetyl-CoA. Both reactions are inhibited by low concentrations of avidin and p-chloromercuribenzoate.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
