{"title":"Electron transport in Peptostreptococcus elsdenii","authors":"R.L. Baldwin, L.P. Milligan","doi":"10.1016/0926-6569(64)90001-X","DOIUrl":null,"url":null,"abstract":"<div><p>The electron-transfer reactions associated with acyl-CoA dehydrogenase (acyl-CoA: DPN<sup>+</sup> oxidoreductase), hydrogenase (EC 1.98.1.1) and lactic dehydrogenase (<span>dl</span>-lactate: (acceptor) oxidoreductase) in the rumen microorganism <em>Peptostreptococcus elsdenii</em> were studied and a pathway of electron transport in the organism proposed. Acyl-CoA dehydrogenase was resolved into dehydrogenase and an associated electron-transport protein (Fraction II) which accepts electrons from DPNH and passes them to the dehydrogenase. The two components were purified and partially characterized. Data indicating the presence of a ferredoxin-linked DPN<sup>+</sup> reductase (reduced ferredoxin: DPN<sup>+</sup> oxidoreductase) are presented.</p></div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 3","pages":"Pages 421-432"},"PeriodicalIF":0.0000,"publicationDate":"1964-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90001-X","citationCount":"34","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/092665696490001X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 34
Abstract
The electron-transfer reactions associated with acyl-CoA dehydrogenase (acyl-CoA: DPN+ oxidoreductase), hydrogenase (EC 1.98.1.1) and lactic dehydrogenase (dl-lactate: (acceptor) oxidoreductase) in the rumen microorganism Peptostreptococcus elsdenii were studied and a pathway of electron transport in the organism proposed. Acyl-CoA dehydrogenase was resolved into dehydrogenase and an associated electron-transport protein (Fraction II) which accepts electrons from DPNH and passes them to the dehydrogenase. The two components were purified and partially characterized. Data indicating the presence of a ferredoxin-linked DPN+ reductase (reduced ferredoxin: DPN+ oxidoreductase) are presented.