Isolation of fucose-rich glycopeptides from normal urine

Abstract

The non-ultrafilterable material from pooled human urine was submitted to block electrophoresis and two fractions relatively rich in fucose but poor in other sugars and in proteins were further fractionated by various means. The material proved to contain a considerable number of fucose-rich glycopeptides, some of which were obtained in a reasonably pure state. The molecular weight was determined in three instances, giving values in the range of 5 000–10 000. Two of the glycopeptides were chemically characterized. One of them had a chemical composition very close to that given for purified blood group antigens. It was serologically inactive.