{"title":"Soluble ribonucleic acid and glycoprotein biosynthesis in the mouse liver","authors":"Hyogo Sinohara, Howard H. Sky-Peck","doi":"10.1016/0926-6534(65)90033-3","DOIUrl":null,"url":null,"abstract":"<div><p>The specific activity of subcellular fractions of liver was determined at varying intervals after the intraperitoneal injection of [1-<sup>14</sup>C]glucosamine in mice. The deoxycholate-soluble portion of microsomal particulates was the most active. During the course of [<sup>14</sup>C]glucosamine incorporation into microsomal fraction <em>in vivo</em>, by way of uridine diphosphate-<em>N</em>-acetylglucosamine, the soluble ribonucleic acids isolated from the high-speed supernatant fraction were not labeled. Soluble ribonucleic acids from ribosomes were unlabeled also. Attempts to incorporate [<sup>14</sup>C]glucosamine into ribonucleic acids <em>in vitro</em> were also unsuccessful. A possible mechanism of carbohydrate addition to polypeptide chains is discussed.</p></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":"101 1","pages":"Pages 90-96"},"PeriodicalIF":0.0000,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90033-3","citationCount":"28","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926653465900333","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 28
Abstract
The specific activity of subcellular fractions of liver was determined at varying intervals after the intraperitoneal injection of [1-14C]glucosamine in mice. The deoxycholate-soluble portion of microsomal particulates was the most active. During the course of [14C]glucosamine incorporation into microsomal fraction in vivo, by way of uridine diphosphate-N-acetylglucosamine, the soluble ribonucleic acids isolated from the high-speed supernatant fraction were not labeled. Soluble ribonucleic acids from ribosomes were unlabeled also. Attempts to incorporate [14C]glucosamine into ribonucleic acids in vitro were also unsuccessful. A possible mechanism of carbohydrate addition to polypeptide chains is discussed.
腹腔注射[1-14C]葡萄糖胺后，以不同的时间间隔测定小鼠肝脏亚细胞组分的比活性。微粒体微粒的脱氧胆碱可溶性部分是最活跃的。在体内[14C]氨基葡萄糖通过尿苷二磷酸- n -乙酰氨基葡萄糖掺入微粒体片段的过程中，高速上清部分分离的可溶性核糖核酸不被标记。核糖体中的可溶性核糖核酸也未标记。在体外将[14C]葡萄糖胺掺入核糖核酸的尝试也没有成功。讨论了碳水化合物加成多肽链的可能机理。
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