Cryopreservation of tissue engineered constructs for bone

IF 2.1 3区 医学 Q2 ORTHOPEDICS Journal of Orthopaedic Research® Pub Date : 2006-01-01 DOI:10.1016/S0736-0266(03)00103-7
Michelle D. Kofron, Natalie C. Opsitnick, Mohamed A. Attawia, Cato T. Laurencin
{"title":"Cryopreservation of tissue engineered constructs for bone","authors":"Michelle D. Kofron,&nbsp;Natalie C. Opsitnick,&nbsp;Mohamed A. Attawia,&nbsp;Cato T. Laurencin","doi":"10.1016/S0736-0266(03)00103-7","DOIUrl":null,"url":null,"abstract":"<p>The large-scale clinical use of tissue engineered constructs will require provisions for its mass availability and accessibility. Therefore, it is imperative to understand the effects of low temperature (–196°C) on the tissue engineered biological system. Initial studies used samples of the osteoblast-like cell line (SaOS-2) adhered to a two-dimensional poly(lactide-<i>co</i>-glycolide) thin film (2D-PLAGA) or a three-dimensional poly(lactide-<i>co</i>-glycolide) sintered microsphere matrix (3D-PLAGA) designed for bone tissue engineering. Experimental samples were tested for their ability to maintain cell viability, following low temperature banking for one week, in solutions of the penetrating cryoprotective agents, dimethylsulfoxide (DMSO), ethylene glycol, and glycerol. Results indicated the DMSO solution yielded the greatest percent cell survival for SaOS-2 cells adhered to both the 2D- and 3D-PLAGA scaffolds; therefore, DMSO was used to cryopreserve mineralizing primary rabbit osteoblasts cells adhered to 2D-PLAGA matrices for 35 days. Results indicated retention of the extracellular matrix architecture as no statistically significant difference in the pre- and post-thaw mineralized structures was measured. Percent cell viability of the mineralized constructs following low temperature storage was approximately 50%. These are the first studies to address the issue of preservation techniques for tissue engineered constructs. The ability to successfully cryopreserve mineralized tissue engineered matrices for bone may offer an unlimited and readily available source of bone-like materials for orthopaedic applications. © 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.</p>","PeriodicalId":16650,"journal":{"name":"Journal of Orthopaedic Research®","volume":"21 6","pages":"1005-1010"},"PeriodicalIF":2.1000,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0736-0266(03)00103-7","citationCount":"33","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Orthopaedic Research®","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1016/S0736-0266%2803%2900103-7","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ORTHOPEDICS","Score":null,"Total":0}
引用次数: 33

Abstract

The large-scale clinical use of tissue engineered constructs will require provisions for its mass availability and accessibility. Therefore, it is imperative to understand the effects of low temperature (–196°C) on the tissue engineered biological system. Initial studies used samples of the osteoblast-like cell line (SaOS-2) adhered to a two-dimensional poly(lactide-co-glycolide) thin film (2D-PLAGA) or a three-dimensional poly(lactide-co-glycolide) sintered microsphere matrix (3D-PLAGA) designed for bone tissue engineering. Experimental samples were tested for their ability to maintain cell viability, following low temperature banking for one week, in solutions of the penetrating cryoprotective agents, dimethylsulfoxide (DMSO), ethylene glycol, and glycerol. Results indicated the DMSO solution yielded the greatest percent cell survival for SaOS-2 cells adhered to both the 2D- and 3D-PLAGA scaffolds; therefore, DMSO was used to cryopreserve mineralizing primary rabbit osteoblasts cells adhered to 2D-PLAGA matrices for 35 days. Results indicated retention of the extracellular matrix architecture as no statistically significant difference in the pre- and post-thaw mineralized structures was measured. Percent cell viability of the mineralized constructs following low temperature storage was approximately 50%. These are the first studies to address the issue of preservation techniques for tissue engineered constructs. The ability to successfully cryopreserve mineralized tissue engineered matrices for bone may offer an unlimited and readily available source of bone-like materials for orthopaedic applications. © 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
骨组织工程构建物的低温保存
组织工程结构的大规模临床应用将需要对其大量可用性和可及性作出规定。因此,了解低温(-196°C)对组织工程生物系统的影响势在必行。最初的研究将成骨细胞样细胞系(SaOS-2)的样品粘附在用于骨组织工程的二维聚(乳酸-羟基乙酸酯)薄膜(2D-PLAGA)或三维聚(乳酸-羟基乙酸酯)烧结微球基质(3D-PLAGA)上。实验样品在低温保存一周后,在穿透性冷冻保护剂二甲基亚砜(DMSO)、乙二醇和甘油的溶液中测试其维持细胞活力的能力。结果表明,DMSO溶液对粘附在2D和3D-PLAGA支架上的SaOS-2细胞的细胞存活率最高;因此,使用DMSO将矿化兔原代成骨细胞粘附在2D-PLAGA基质上冷冻保存35天。结果表明,细胞外基质结构的保留在解冻前和解冻后的矿化结构中没有统计学上的显著差异。低温储存后,矿化构建体的细胞存活率约为50%。这些是解决组织工程构建的保存技术问题的第一个研究。成功低温保存矿化组织工程骨基质的能力可能为骨科应用提供无限且容易获得的骨样材料来源。©2003骨科研究学会。Elsevier Science Ltd.出版。版权所有。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of Orthopaedic Research®
Journal of Orthopaedic Research® 医学-整形外科
CiteScore
6.10
自引率
3.60%
发文量
261
审稿时长
3-6 weeks
期刊介绍: The Journal of Orthopaedic Research is the forum for the rapid publication of high quality reports of new information on the full spectrum of orthopaedic research, including life sciences, engineering, translational, and clinical studies.
期刊最新文献
The LINC Complex Regulates Tendon Elastic Modulus, Collagen Crimp, and Lateral Expansion During Early Postnatal Development. Grip Force Modulation on Median Nerve Morphology Changes. Issue Information - Cover Issue Information - Editorial Board and TOC Generating New Meniscus Therapies via Recent Breakthroughs in Development, Model Systems, and Clinical Diagnostics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1