Halim Nagem-Filho, César Roberto Monteiro, Haline Drumond Nagem, José Luiz Lage-Marques
{"title":"Effect of dental adhesives on the exudative phase of the inflammatory process in subcutaneous tissue of rats.","authors":"Halim Nagem-Filho, César Roberto Monteiro, Haline Drumond Nagem, José Luiz Lage-Marques","doi":"10.1590/s1517-74912003000200002","DOIUrl":null,"url":null,"abstract":"<p><p>The vascular changes in the subcutaneous connective tissue of rats induced by dentin bonding systems (one step) was studied and compared to those induced by saline solution (negative control) and Furacin (positive control), during the exudative phase of the inflammatory process. Twenty mg/kg of Evan's blue were injected intravenously in the vein of the rats' penises; 0.1 ml of each substance tested was inoculated in the subcutaneous tissue. After a 3 hour period the animals were sacrificed and their skins were excised and punched out with a standard steel 2.5 cm in diameter. The specimens were immediately immersed in 8 ml of formamide and taken to a double boiler for 72 hours at 37 C, to remove the dye. The liquid containing the overflowed dye was filtered, analyzed in the spectrophotometer (620 nm) and classified according to the criteria established by Nagem-Filho, Pereira (1976). After statistical analysis, the irritative potential of the substances was ranked as follows: Furacin (severe) > Single Bond and Bond 1 (moderate - no significant differences between the dentin bonding systems tested) > saline solution (not significant as regards the irritation degree).</p>","PeriodicalId":35295,"journal":{"name":"Pesquisa odontologica brasileira = Brazilian oral research","volume":"17 2","pages":"109-12"},"PeriodicalIF":0.0000,"publicationDate":"2003-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/s1517-74912003000200002","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesquisa odontologica brasileira = Brazilian oral research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/s1517-74912003000200002","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2003/10/10 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Dentistry","Score":null,"Total":0}
引用次数: 10
Abstract
The vascular changes in the subcutaneous connective tissue of rats induced by dentin bonding systems (one step) was studied and compared to those induced by saline solution (negative control) and Furacin (positive control), during the exudative phase of the inflammatory process. Twenty mg/kg of Evan's blue were injected intravenously in the vein of the rats' penises; 0.1 ml of each substance tested was inoculated in the subcutaneous tissue. After a 3 hour period the animals were sacrificed and their skins were excised and punched out with a standard steel 2.5 cm in diameter. The specimens were immediately immersed in 8 ml of formamide and taken to a double boiler for 72 hours at 37 C, to remove the dye. The liquid containing the overflowed dye was filtered, analyzed in the spectrophotometer (620 nm) and classified according to the criteria established by Nagem-Filho, Pereira (1976). After statistical analysis, the irritative potential of the substances was ranked as follows: Furacin (severe) > Single Bond and Bond 1 (moderate - no significant differences between the dentin bonding systems tested) > saline solution (not significant as regards the irritation degree).
期刊介绍:
Pesquisa Odontológica Brasileira is a new quarterly published journal (January-March, April-June, July-September, October-December), with an annual supplement (Anais da Reunião de Pesquisa Odontológica da SBPqO), by the Sociedade Brasileira de Pesquisa Odontológica [Brazilian Society of Odontological Research] and University of São Paulo. It replaces Revista de Odontologia da Universidade de São Paulo (ISSN 0103-0663).