Effect of dental adhesives on the exudative phase of the inflammatory process in subcutaneous tissue of rats.

Halim Nagem-Filho, César Roberto Monteiro, Haline Drumond Nagem, José Luiz Lage-Marques
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引用次数: 10

Abstract

The vascular changes in the subcutaneous connective tissue of rats induced by dentin bonding systems (one step) was studied and compared to those induced by saline solution (negative control) and Furacin (positive control), during the exudative phase of the inflammatory process. Twenty mg/kg of Evan's blue were injected intravenously in the vein of the rats' penises; 0.1 ml of each substance tested was inoculated in the subcutaneous tissue. After a 3 hour period the animals were sacrificed and their skins were excised and punched out with a standard steel 2.5 cm in diameter. The specimens were immediately immersed in 8 ml of formamide and taken to a double boiler for 72 hours at 37 C, to remove the dye. The liquid containing the overflowed dye was filtered, analyzed in the spectrophotometer (620 nm) and classified according to the criteria established by Nagem-Filho, Pereira (1976). After statistical analysis, the irritative potential of the substances was ranked as follows: Furacin (severe) > Single Bond and Bond 1 (moderate - no significant differences between the dentin bonding systems tested) > saline solution (not significant as regards the irritation degree).

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牙粘接剂对大鼠皮下炎症过程渗出期的影响。
研究了牙本质结合系统(一步法)诱导的大鼠皮下结缔组织血管变化,并与生理盐水(阴性对照)和呋喃星(阳性对照)诱导的大鼠皮下结缔组织血管变化进行了比较。在大鼠阴茎静脉内静脉注射埃文蓝20 mg/kg;将每种被试物质分别接种于皮下组织0.1 ml。3小时后,动物被处死,他们的皮肤被切除,用直径2.5厘米的标准钢打孔。立即将标本浸入8ml甲酰胺中,并在37℃的双锅中放置72小时,以去除染料。将含有溢出染料的液体过滤,用分光光度计(620 nm)分析,并根据Nagem-Filho, Pereira(1976)建立的标准进行分类。经统计分析,各物质的刺激电位排序为:Furacin(严重)> Single Bond和Bond 1(中等-测试的牙本质结合系统之间无显著差异)>生理盐水溶液(刺激程度无显著差异)。
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来源期刊
CiteScore
3.80
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0.00%
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期刊介绍: Pesquisa Odontológica Brasileira is a new quarterly published journal (January-March, April-June, July-September, October-December), with an annual supplement (Anais da Reunião de Pesquisa Odontológica da SBPqO), by the Sociedade Brasileira de Pesquisa Odontológica [Brazilian Society of Odontological Research] and University of São Paulo. It replaces Revista de Odontologia da Universidade de São Paulo (ISSN 0103-0663).
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