Ampicillin resistance in Listeria monocytogenes acquired as a result of transposon mutagenesis.

Acta microbiologica Polonica Pub Date : 2003-01-01
Joanna Poroś-Głuchowska, Małgorzata Kłoszewska, Zdzisław Markiewicz
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Abstract

We have used plasmid pLTV3, which carries transposon Tn917, to obtain a series of mutants of Listeria monocytogenes EGD showing varied degrees of resistance to ampicillin and other beta-lactam antibiotics, including imipenem. In this paper we focus on the characteristics of two strains in which decreased susceptibility to ampicillin is accompanied by changes in the structure of the cell wall murein and cell-wall related changes of phenotype.

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转座子诱变所致单核增生李斯特菌氨苄西林耐药性的研究。
我们利用携带转座子Tn917的质粒pLTV3获得了一系列单核增生李斯特菌EGD突变体,这些突变体对氨苄西林和其他β -内酰胺类抗生素(包括亚胺培南)具有不同程度的耐药性。在本文中,我们重点研究了两种菌株的特点,其中对氨苄西林的敏感性降低伴随着细胞壁结构的变化和细胞壁相关表型的变化。
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