Tissue transglutaminase expression in duodenal mucosa of patients with celiac disease and of normal subjects.

IF 1.6 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY Turkish Journal of Gastroenterology Pub Date : 2003-09-01

Ilyas Tuncer, Irfan Bayram, Ismet Kaba, Ridvan Mercan, Serdar Uğraş

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引用次数: 0

Abstract

Background/aims: Our aim in the present study was to investigate tissue transglutaminase expression by immunohistochemistry in duodenal mucosa of patients with celiac disease.

Methods: A total of twelve patients with celiac disease were examined. The patients had different clinical and histopathologic degrees of severity and responded to gluten withdrawal with clinical improvement. Ten subjects with various unrelated diseases served as controls. Serum endomysium antibodies were measured by an indirect immunofluorescence method using a commercial kit. Duodenal biopsy specimens were stained with hematoxylin and eosin. Immunohistochemical staining for anti-tissue transglutaminase antibodies was performed using commercial kit.

Results: Serum endomysium antibodies and evaluation of small bowel biopsy specimens were normal in all control subjects. However, serum endomysium antibodies were positive in all of the celiac patients. Immunohistochemical staining pattern of duodenal biopsy specimen performed using anti-tissue transglutaminase antibodies was similar in celiac patients and control subjects.

Conclusion: Tissue transglutaminase expression by immunohistochemical methods in untreated celiac mucosa is not suitable for diagnosis of celiac disease.

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乳糜泻患者与正常人十二指肠黏膜组织转谷氨酰胺酶的表达。

背景/目的:本研究的目的是通过免疫组织化学方法研究乳糜泻患者十二指肠黏膜组织转谷氨酰胺酶的表达。方法:对12例乳糜泻患者进行检查。患者有不同的临床和组织病理学严重程度，并对谷蛋白戒断有反应，临床改善。10名患有各种不相关疾病的受试者作为对照。血清肌内膜抗体采用间接免疫荧光法测定。十二指肠活检标本用苏木精和伊红染色。使用商业试剂盒进行抗组织转谷氨酰胺酶抗体的免疫组化染色。结果:对照组血清肌内膜抗体及小肠活检结果均正常。然而，所有乳糜泻患者血清肌内膜抗体均呈阳性。使用抗组织转谷氨酰胺酶抗体进行十二指肠活检标本的免疫组织化学染色模式在乳糜泻患者和对照组中相似。结论:免疫组化法在未治疗的乳糜泻黏膜组织中表达转谷氨酰胺酶不适合诊断乳糜泻。

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来源期刊

Turkish Journal of Gastroenterology 医学-胃肠肝病学

CiteScore

1.90

自引率

0.00%

发文量

127

审稿时长

6 months

期刊介绍： The Turkish Journal of Gastroenterology (Turk J Gastroenterol) is the double-blind peer-reviewed, open access, international publication organ of the Turkish Society of Gastroenterology. The journal is a bimonthly publication, published on January, March, May, July, September, November and its publication language is English. The Turkish Journal of Gastroenterology aims to publish international at the highest clinical and scientific level on original issues of gastroenterology and hepatology. The journal publishes original papers, review articles, case reports and letters to the editor on clinical and experimental gastroenterology and hepatology.