Prevalence of antinuclear autoantibodies in the serum of normal blood dornors.

Revista do Hospital das Clinicas Pub Date : 2003-11-01 Epub Date: 2004-01-28 DOI:10.1590/s0041-87812003000600005

Solange Assuncion Villagra Fernandez, Alice Zoghbi Coelho Lobo, Zilda Najjar Prado de Oliveira, Ligia Maria Ichimura Fukumori, Alexandre Marques P rigo, Evandro A Rivitti

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引用次数: 83

Abstract

Objective: To examine the presence of serum antinuclear autoantibodies in a healthy population.

Methods: Serum of 500 normal blood donors between 18 and 60 years of age were tested for the presence of autoantibodies. Antinuclear antibodies were detected by indirect immunofluorescence technique using HEp-2 epithelial cells as the substrate. The presence of dnaN was detected by indirect immunofluorescence technique using Critidia lucillae as the substrate. Anti-SSA (RO), anti-SSB (LA), anti-Sm, and anti-RNP were determined by double radial immunodiffusion.

Results: In the evaluation of the presence of serum antibodies, antinuclear antibodies were detected in 22.6% of the sera. The presence of other antibodies was not significant. The majority of the titers were 1:40.

Conclusion: The presence of autoantibodies is not necessarily pathologic and has to be related to the age group, gender, and clinical condition of the patient.

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正常供血者血清中抗核自身抗体的流行。

目的:检测健康人群血清抗核自身抗体的存在。方法:对500例18 ~ 60岁的正常献血者进行血清自身抗体检测。以HEp-2上皮细胞为底物，采用间接免疫荧光技术检测抗核抗体。采用间接免疫荧光技术，以透明荆芥为底物检测dnaN的存在。双径向免疫扩散法检测抗ssa (RO)、抗ssb (LA)、抗sm和抗rnp。结果:在血清抗体检测中，抗核抗体检出率为22.6%。其他抗体的存在不显著。大多数滴度为1:40。结论:自身抗体的存在不一定是病理性的，与患者的年龄、性别和临床状况有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Revista do Hospital das Clinicas

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