Down regulation of DRET gene in human hepatocellular carcinoma tissues samples from Qidong area, China.

Eksperimental'naia onkologiia Pub Date : 2004-03-01

Dong-Dong Lu, Xi-Ran Zhang

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Abstract

Objective: To analyze the expression of DRET gene in hepatocellular carcinomas (HCCs) tissue specimens in comparison with normal liver tissue to evaluate the relationship between DRET gene and HCC.

Methods: 250 primary HCCs and 50 normal liver tissue samples from Qidong area, China, were studied for DRET mRNA and protein expression with the use of Northern blot analysis, in situ hybridization and immunohistochemistry.

Results: By Northern analysis, moderate to strong DRET mRNA expression was present in normal liver samples. In contrast, DRET mRNA expression in tissue samples of primary HCCs was markedly decreased compared with normal controls. Primary HCCs that gave rise to metastasis showed significantly lower DRET mRNA levels than nonmetastasizing HCCs. By in situ hybridization analysis, nonmetastatic HCCs samples didn't differ from controls. In contrast, most of the primary metastasizing HCC showed only faint or moderate DRET mRNA expression. Tissue sections of nonmetastatic HCC exhibited lower DRET immunoreactivity than control samples, but higher labeling index than metastatic HCC samples.

Conclusions: Expression of DRET on mRNA and protein levels in HCC cells is related to HCC metastatic ability.

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启东地区人肝癌组织中DRET基因的下调

目的:分析DRET基因在肝细胞癌(HCC)组织标本中的表达，并与正常肝组织进行比较，探讨DRET基因与HCC的关系。方法:采用Northern blot、原位杂交和免疫组化方法，对启东地区250例原发性hcc和50例正常肝组织进行DRET mRNA和蛋白的表达研究。结果:通过Northern分析，正常肝脏样本中存在中强DRET mRNA表达。相比之下，与正常对照相比，原发性hcc组织样本中的DRET mRNA表达明显降低。引起转移的原发性hcc的DRET mRNA水平明显低于非转移性hcc。通过原位杂交分析，非转移性hcc样本与对照组没有差异。相比之下，大多数原发性转移性HCC仅显示微弱或中度的DRET mRNA表达。非转移性HCC组织切片的DRET免疫反应性低于对照样本，但标记指数高于转移性HCC样本。结论:肝癌细胞中DRET mRNA和蛋白水平的表达与肝癌转移能力有关。

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Eksperimental'naia onkologiia

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