Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene.

Song-Heng Jin, De-An Jiang, Xue-Qin Li, Jun-Wei Sun
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反义Rubisco激活酶基因转化水稻植株的光合特性。
利用农杆菌插入反义基因的转基因水稻植株,研究Rubisco激活酶(RCA)的降低对Rubisco和光合作用的影响。在本研究中,选择了含有15% ~ 35%野生型Rubisco激活酶的转化子,该转化子可以在环境CO2浓度下存活,但与野生型对照相比生长缓慢。气体交换测量结果表明,光合速率显著降低,气孔导度和蒸腾速率没有变化;细胞间的二氧化碳浓度甚至增加了。Rubisco测定表明,这些植物的Rubisco含量大约是野生型的两倍,尽管它们的光合作用速率降低了70%,这可能是对Rubisco激活酶和/或氨甲酰化降低的驯化反应。
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