The diagnostic significance of the detection of cytokeratin 19 mRNA by quantitative RT-PCR in benign and malignant pleural effusions.

Journal of Zhejiang University. Science Pub Date : 2004-10-01 DOI:10.1631/jzus.2004.1286

Feng Xu, Jie Chen, Hua-hao Shen, Xuan-ding Wang, Jiang Shan

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引用次数: 3

Abstract

Objective: To evaluate the diagnostic significance of detecting cytokeratin 19 (CK19) mRNA by quantitative reverse transcription polymerase chain reaction (RT-PCR) in benign and malignant pleural effusions.

Methods: CK19 mRNA was examined by quantitative RT-PCR and CK19 was detected by Enzyme-linked immunoadsorbent assay (ELISA) in 32 patients with malignant pleural effusions and 35 patients with benign pleural effusions.

Results: On the threshold of 200 copies/microl, the positive rate of CK19 mRNA in patients with malignant pleural effusions was 62.5%. The positive rates of CK19 mRNA and CK19 in the malignant pleural effusions were significantly higher than those in the benign group (P<0.01). Furthermore, the positive rate of CK19 mRNA was higher than that of CK19 in the malignant group (P<0.05).

Conclusion: Detection of CK19 mRNA can be a promising diagnostic marker in differential diagnosis of benign and malignant pleural effusions.

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定量RT-PCR检测细胞角蛋白19 mRNA在良、恶性胸腔积液中的诊断意义。

目的:探讨定量逆转录聚合酶链反应(RT-PCR)检测细胞角蛋白19 (CK19) mRNA在良、恶性胸腔积液中的诊断意义。方法:采用定量RT-PCR检测32例恶性胸腔积液患者的CK19 mRNA，采用酶联免疫吸附法(ELISA)检测35例良性胸腔积液患者的CK19。结果:在200拷贝/微l阈值下，CK19 mRNA在恶性胸腔积液患者中的阳性率为62.5%。CK19 mRNA和CK19在恶性胸腔积液中的阳性率明显高于良性组(p结论:CK19 mRNA的检测可作为良恶性胸腔积液鉴别诊断的一个有希望的诊断指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Zhejiang University. Science

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